Thermo Scientific™ aLICator LIC Cloning and Expression Kit 3 (C-terminal His-tag)

Código de producto. 11901421

Marca: Thermo Scientific™ K1261

Descripción

Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.

aLICator LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in E. coli. The pLATE bacterial expression vectors are designed for high levels of target protein expression in concert with minimal background (uninduced) expression, which permits expression of proteins that are toxic to E. coli cells. To streamline and facilitate the process of insert cloning into the expression vector, the aLICator system uses directional LIC cloning technology, a rapid procedure that provides high-cloning efficiencies.

The tightly regulated expression and fast, efficient directional cloning makes the aLICator LIC Cloning and Expression System the best choice for routine and toxic gene cloning and expression in E. coli.

Highlights

• High efficiency LIC cloning
• Tight control of gene expression
• High yield expression
• Versatile -tagged or untaged protein expression with tag removal option

Especificaciones

• Antibiotic Resistance Bacterial: Ampicillin (AmpR)
• Cloning Method: LIC Cloning
• Format: Kit
• Sample Type: PCR Products
• For Use With (Application): PCR Cloning
• No. of Reactions: 20 Reactions
• Product Line: aLICator
• Product Type: LIC Cloning and Expression Kit
• Promoter: T7
• Protein Tag: His Tag (6x)
• Quantity: 20 Reactions
• Vector: pLATE

Seguridad y manipulación

Nombre del producto

• aLICator LIC Cloning and Expression Kit 3 (C-terminal His-tag)

Palabra de advertencia

• Warning

Clasificación

• Specific target organ toxicity after repeated exposure Category 2

Indicaciones de peligro

• H373-May cause damage to organs.

Consejos de prudencia

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P314-Get medical advice/attention if you feel unwell.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Otros peligros

• MIXTURE LIST-Contains : Glycine, N,N-1,2-ethanediylbis[N-(carboxymeth yl)-, disodiumsalt, dihydrate

For Research Use Only. Not for use in diagnostic procedures.