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Invitrogen™ Bolt™ Antioxidant
A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Thermo Scientific™ Pierce™ Horseradish Peroxidase
Purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
| Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C. |
|---|---|
| Form | Powder |
| Label or Dye | HRP (Horseradish Peroxidase) |
| For Use With (Application) | Western Blotting, ELISA, Immunohistochemistry |
| Product Line | Pierce™ |
Thermo Scientific™ Pierce™ 1-Step Transfer Buffer
Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.
| Product Type | Transfer Buffer |
|---|
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
| Shipping Condition | Room Temperature |
|---|---|
| Substrate | Horseradish Peroxidase |
| Recommended Antibody Concentrations | 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL) |
| Conjugate | HRP |
| Form | Liquid |
| Product Type | Substrate |
| Signal Duration | 6 to 24 hr. |
| For Use With (Application) | Western Blot |
| Product Line | SuperSignal™ |
| Detection Method | Chemiluminescence |
Invitrogen™ iBind™ Flex Fluorescent Detection (FD) Solution Kit
Detection kit for use with iBind™ Flex Western System
Thermo Scientific™ EZ-Link™ Maleimide Activated Horseradish Peroxidase
Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.
Invitrogen™ Amplex™ Red and Amplex™ UltraRed Reagents
The Amplex Red and UltraRed reagents are fluorogenic substrates for peroxidase. These highly sensitive and stable probes react with H2O2 in the presence of HRP for solution-based assays and ELISAs. Amplex UltraRed is a brighter version of Amplex Red with an improved pH range and sensitivity.
| Substrate | HRP (Horseradish Peroxidase) Substrate |
|---|---|
| Shipping Condition | Room Temperature |
| Content And Storage | Store in freezer (-5°C to -30°C). |
| Form | Solid |
| Product Type | Red Hydrogen Peroxide |
| For Use With (Equipment) | Fluorometer,Microplate Reader |
| Validated Application | Cell Analysis |
| For Use With (Application) | Hydrogen Peroxidase Assay |
| Product Line | Amplex |
| Detection Method | Fluorescence |
Invitrogen™ PFB-FDGlu (5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside)
PFB-FDGlu is a cell-permeable substrate used for live cell imaging of GCase activity. It fluoresces green upon cleavage by lysosomal GCase, facilitating specific, single-cell analysis via high-content imaging, flow cytometry, and microscopy.
Thermo Scientific™ Clean-Blot™ IP Detection Reagent (HRP)
Avoid interference from denatured IP antibody fragments in Western blots of immunoprecipitated proteins with these HRP and AP conjugates.
| No. of Reactions | 10 midi blots or 20 mini blots |
|---|---|
| Product Type | Flex Solution Kit |
| For Use With (Equipment) | iBind Flex Western Device |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Product Line | iBind™ |
| Detection Method | Colorimetric, Chromogenic, Chemiluminescence |
Thermo Scientific™ beta-Galactosidase Assay Reagent
Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.
| Substrate | Beta-Gal Substrate |
|---|---|
| Assay | Reporter Enzyme |
| Substrate Properties | Chemical Substrate |
| Content And Storage | Store at -20°C. |
| Format | 96-well Plate |
| Product Type | Pierce beta-Galactosidase Assay Reagent |
| For Use With (Equipment) | Microplate Reader |
| Form | Liquid |
| Label or Dye | Enzyme Labeled |
| Target Enzyme | Beta-Galactosidase |
| For Use With (Application) | Absorbance |
| Product Line | Pierce |
| Detection Method | Colorimetric |
Thermo Scientific™ SuperSignal™ Western Blot Enhancer
Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.
| Content And Storage | Upon receipt store solutions at 4°C Products are shipped at ambient temperature. |
|---|---|
| Form | Liquid |
| Product Type | Western Blot Enhancer |
| Membrane Compatibility | Nitrocellulose, PVDF |
| For Use With (Application) | Western Blot |
| Product Line | SuperSignal™ |
| Detection Method | Chemiluminescence, Fluorescence, Colorimetric |
Thermo Scientific™ Pierce™ High Sensitivity NeutrAvidin™-HRP
Specially prepared peroxidase-conjugated NeutrAvidin protein for maximum signal amplification with high signal-to-noise ratio in a ready-to-use solution.
| Specificity | Biotin |
|---|---|
| Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2°C to 8°C. |
| Target Molecule | Biotin |
| Form | Liquid |
| Enzyme | HRP (Horseradish Peroxidase) |
| Label or Dye | HRP (Horseradish Peroxidase) |
| For Use With (Application) | Western Blotting, ELISA |
| Product Line | Pierce™ |
| Detection Method | Colorimetric, Chemiluminescence, Chemifluorescence |
Thermo Scientific™ Pierce™ High Sensitivity Streptavidin-HRP, Pre-Diluted
Amplify signal in Western blot, ELISA or other biotin-based detection methods with this exclusive, high signal-to-noise, peroxidase-conjugated, ready-to-use streptavidin.