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Thermo Scientific™ Pierce™ Maleimide-Activated HRP Conjugation Kit

Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.

Brand:  Thermo Scientific™ 31485

UNSPSC : 12352204

Code : PN

Additional Details : CAS Number : 22590-64-3 Weight : 1.81440kg

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Product Code. 11839150

  • £192.00 / 5mg


Estimated Shipment
06-10-2017
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Description

Description

The Thermo Scientific Maleimide Activated Horseradish Peroxidase (HRP) Kit can be used to directly prepare HRP conjugates from proteins, peptides or other ligands that contain a free sulfhydryl group. This kit eliminates the first step of the two-step maleimide method, making conjugate preparation much simpler. The kit is supplied with a stable, Maleimide-activated horseradish peroxidase. HRP conjugates can be purified by gel filtration chromatography, ultrafiltration or dialysis, depending upon the size of the conjugated protein.

Highlights:

  • Prepare enzyme conjugates from proteins that contain a free sulfhydryl
  • Includes 2-MEA to generate free sulfhydryls from disulfide bonds
  • Includes SATA to add free sulfhydryls to lysine residues
Specifications

Specifications

5mg
Maleimide Act. Hrp 5mg/pk
Activated horseradish peroxidase lyophilized from PBS
Conjugation with approx. 5mg of antibody (IgG)