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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (263)
Real Time PCR Reagents and Kits (206)
Real Time PCR Assays and Arrays (98)

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1
Promotions Available

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 25 X
Description Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase ArrayScript™
Concentration 25X
PCR Method 1-step RT-qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Product Type One-Step RT-PCR Reagent
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Sample Type RNA
Reaction Speed Standard
Product Line AgPath-ID, Ambion
2

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

GC-Rich PCR Performance High
Fidelity (vs. Taq) 25 X
Description Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase ArrayScript™
Concentration 25X
PCR Method 1-step RT-qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Product Type One-Step RT-PCR Reagent
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 System
Reaction Speed Standard
Product Line AgPath-ID, Ambion
3

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

EDGE
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Product Type Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
4

Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG

Is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2).

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Product Type Quantitative PCR SuperMix-UDG
For Use With (Equipment) 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Platinum
Detection Method Primer-probe
5
Promotions Available

Applied Biosystems™ TaqMan™ RNase P Detection Reagents Kit

TaqMan™ RNase P Detection Reagents Kit

EDGE
6
Promotions Available

Applied Biosystems™ Power SYBR™ Green PCR Master Mix

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

EDGE
GC-Rich PCR Performance High
Purity or Quality Grade LD (Low DNA)
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method SYBR
Polymerase AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Label or Dye SYBR Green
Product Line SYBR
7
Promotions Available

Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG

TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.

EDGE
Shipping Condition Wet Ice
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Product Type Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
8
Promotions Available

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

EDGE
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq DNA Polymerase
Format Tube
Product Type Fast Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Product Line AmpErase, TaqMan
9
Promotions Available

Applied Biosystems™ Absolute Q™ Universal DNA Digital PCR Master Mix (5X)

Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned TaqMan Assays, eliminating the need to design custom assays.

EDGE
10

Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents
GREENER_CHOICE

TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.

GC-Rich PCR Performance Low
Format Tube(s)
Internal Probe Modification TAMRA™ Quencher (3'), VIC™ (5')
Product Type Exogenous Internal Positive Control Reagent
Reaction Speed Standard
PCR Method qPCR
Product Line TaqMan
11
Promotions Available

Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.

Shipping Condition Dry Ice
Polymerase Taq DNA Polymerase
Product Type One-Step qRT-PCR Kit
For Use With (Equipment) 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Sample Type RNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Pathogen Detection,Virus Detection
PCR Method 1-step RT-qPCR
Product Line Platinum, SuperScript
Detection Method Primer-probe
12

Applied Biosystems™ Detection Enhancer, for real-time PCR

Formulated to improve template amplification in real-time PCR (qPCR) workflows

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Product Type Detection Enhancer
For Use With (Equipment) 7500 System
Sample Type Viral Samples
Reaction Speed Standard
For Use With (Application) Real Time PCR (qPCR)
PCR Method 1-step RT-qPCR
Product Line Applied Biosystems
Detection Method Primer-probe
15
Promotions Available

Invitrogen™ EXPRESS One-Step Superscript™ qRT-PCR Kit, universal

Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.

Polymerase Taq DNA Polymerase
Format Tube
Product Type qRT-PCR Kit
For Use With (Equipment) 7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ
Sample Type RNA
Reaction Speed Fast or Standard
PCR Method 1-step RT-qPCR
Product Line SuperScript
Detection Method Primer-probe