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Chromatography Media

Resins or particles used to separate components of a sample via column chromatography; products include media used in cation or anion exchange, fast flow, and high-performance chromatography techniques, etc.
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1

Cytiva Sephaorose™ Fast Flow Anion Exchange Media: DEAE

Used for preparative protein separations in both research and industrial applications. Cytiva Life Sciences™ Sephaorose™ Fast Flow Anion Exchange Media: DEAE ensures low levels of non-specific binding.

2

Cytiva Butyl-S Sepharose™ 6 Fast Flow Chromatography Media

The least hydrophobic medium in the Sepharose Fast Flow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of purification process

3

Cytiva Capto™ DEAE Chromatography Media

Used for fast, efficient capture and intermediate protein purification

EDGE
4

Applied Biosystems™ Trifluoroacetic Acid (TFA) Neat Liquid

Trifluoroacetic Acid (TFA, concentrated) for use with OPC™ cartridges for oligonucleotide purification.

5

Cytiva HiTrap™ TALON™ Crude Prepacked Columns

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Particle Size 60 to 160 μm
Ligand Type Talon Superflow Cobalt-IMAC
Flow Rate 1 to 4 mL/min
Matrix 6% Cross-Linked Agarose
Storage Buffer 20% Ethanol
pH Range 3 to 12
Product Type HiTrap Talon crude prepacked Column
Material Polypropylene
Storage Requirements 4°C to 8°C
For Use With (Application) For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH
Max. Pressure 3 bar
6

Cytiva DEAE Sepharose™ Fast Flow Chromatography Media

For preparative protein separations in both research and industrial applications. Cytiva Life Sciences™ DEAE Sepharose™ Fast Flow Chromatography Media can be used for capture and intermediate purification of wide range of approved biopharmaceuticals.

7

Cytiva Q Sepharose™ High Performance Chromatography Media

Designed for high intermediate and polishing steps in downstream processing

8

Cytiva His SpinTrap™ TALON™

Single-use spin column for high purity purification of histidine-tagged proteins

9

Cytiva Sepharose™ Fast Flow Cation Exchange Media

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Particle Size 45 to 165 μm
pH Range 4 to 13
10

Cytiva TALON™ Superflow Medium

Offers different selectivity compared to nickel-charged media. Cytiva TALON™ Superflow Medium used for purification of histidine-tagged recombinant proteins by IMAC.

Particle Size 60 to 160 μm
Ligand Type Talon Superflow Cobalt-IMAC
Matrix 6% Cross-Linked Agarose
Storage Buffer 20% Ethanol
pH Range 3 to 12
Product Type Talon Superflow Media
Binding Capacity Up to 20 mg Histidine-tagged Protein
Storage Requirements 4°C to 8°C
For Use With (Application) For PurifiCatIon of Histidine-Tagged Recombinant Pby Immobilized Metal Affinity Chromatography
11

Cytiva SP Sepharose™ High Performance Media

Use for high intermediate and polishing steps in downstream processing. Cytiva Life Sciences™ SP Sepharose™ High Performance Media is composed of crosslinked agarose beads.

12

Cytiva Phenyl Sepharose™ 6 Fast Flow Chromatography Media

Used for capture and intermediate purification requiring low to medium hydrophobicity

Ligand Type Phenyl
Matrix 6% Cross-Linked Agarose
pH Range 3 to 13
For Use With (Application) For Capture and Intermediate PurifiCatIon Requiring Low to Medium Hydrophobicity
13

Cytiva Capto™ Multimodal (MMC) Chromatography Media

Used to solve specific purification challenges at both high and low ionic strengths

Particle Size 75 μm
Product Type Capto MMC Media
14

Cytiva rProtein A Sepharose™ Fast Flow Affinity Media

Designed for purification of monoclonal and polyclonal antibodies at labs and process scales

Particle Size 60 to 165 μm
Ligand Type Recombinant Protein A (E. coli)
Flow Rate 150 to 250 cm/Hoμr
Matrix 4% Cross-Linked Agarose
Stability Stable in aqueous Buffers commonly used in Protein A Chromatography
Storage Buffer 20% Ethanol
pH Range 2 to 9
Product Type rProtein A Sepharose Fast Flow Media
Height (English) Bed 9.84 in.
Storage Requirements 2°C to 8°C
For Use With (Application) For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales
Height (Metric) Bed 25 cm
Max. Pressure 100 kPa
15

Cytiva SOURCE™ 15S Chromatography Media

Chemically stable cation exchanger for high resolution polishing purification of proteins, peptides and oligonucleotides

Stability Stable in common Ion Exchange Buffers
Storage Buffer 20% Ethanol, 0.2 M Sodium Acetate
pH Range 1 to 14
Height (English) Bed 3.93 in.
Storage Requirements 4°C to 30°C
For Use With (Application) For High Resolution Polishing PurifiCatIon of Proteins, Peptides and Oligonucleotides
Max. Pressure 100 kPa
Particle Size 15 μm
Ligand Type Sulphonate Group
Flow Rate 400 cm/Hoμr
Matrix Polystyrene/Divinylbenzene
Product Type Source 15 S Media
Ion Exchange Type Strong CatIon Exchanger
Height (Metric) Bed 10 cm