Binding up to 180mg of His-tagged protein per column
The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.
HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.
High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin
Versatile – purify proteins using native or denaturing conditions
Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges
Cost-effective – reuse the same batch of resin at least five times
Easy to use – pre-formulated buffers available for kit formats