Gel Filtration Media and Columns
A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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Cytiva Sephadex™ G-25 Media
Use of Sephadex™ is well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.
| Height (Metric) | 30cm |
|---|---|
| Content And Storage | 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH |
| Bed Volume | 4 to 6mL/g in Distilled Water |
| Format | Solid Spherical Bead |
| pH Range | 2 to 13 |
| Product Type | Sephadex G-25 Media |
| Diameter (Metric) Inner | 2.6cm |
| For Use With (Application) | Desalting and Buffer exchange and removal free label in industrial applications |
| Support Type | Cross-linked Dextran |
| Height (English) | 11.81 in. |
Cytiva Sephadex™ G10 Media
Gel filtration medium for desalting and buffer exchange of peptides and small biomolecules
| Height (Metric) | 30cm |
|---|---|
| Content And Storage | 4 to 30°C |
| Bed Volume | 2 to 3mL/g in Distilled Water |
| pH Range | 2 to 13 |
| Diameter (Metric) Inner | 2.6cm |
| For Use With (Application) | Desalting and Buffer exchange of Peptides and small biomolecules >700 M.W. |
| Max. Pressure | 24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d |
| Particle Size | 55 to 165 μm in wet form, 40 to 120 μm in dry form |
| Flow Rate | < Darcy´s Law |
| Format | Solid Bead |
| Product Type | Sephadex G10 Media |
| Sample Type | Peptides, Small Biomolecules |
| Support Type | Cross-linked Dextran |
| Height (English) | 11.81 in. |
Cytiva Sephadex™ G-100 Media
Commonly used for desalting and buffer exchange of very large biomolecules
| Content And Storage | 4° to 30°C |
|---|---|
| Bed Volume | 15 to 20mL/g in Distilled Water |
| Format | Solid Bead |
| pH Range | 2 to 10 |
| Product Type | Sephadex G-100 Media |
| Sample Type | Large biomolecules |
| Quantity | 100 g |
| For Use With (Application) | Useful for desalting and Buffer exchange of very large biomolecules |
| Support Type | Cross-linked Dextran |
| Max. Pressure | 0.096 bar |
Cytiva Sephadex™ G15 Media
Gel filtration medium for desalting and buffer exchange for small biomolecules greater than 1500 molecular weight