Crosslinking, Labeling and Protein Modification
Invitrogen™ Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 647 is a bright and photostable far-red dye with excitation ideally suited to the 633 nm laser line.
Invitrogen™ Alexa Fluor™ 647 C2 Maleimide
Alexa Fluor™ 647 is a bright, far-red fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines.
Thermo Scientific™ Trypsin, TPCK Treated
Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.
Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin
Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.
Thermo Scientific™ DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)
Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).
Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.
Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/μL)
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
Invitrogen™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line.
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Thermo Scientific™ EGS (ethylene glycol bis(succinimidyl succinate))
Crosslink amines to amines with this membrane permeable crosslinker composed of NHS-ester groups and a 16.1A hydroxylamine-cleavable spacer arm.
Invitrogen™ Acryloyl-X, SE (6-((acryloyl)amino)hexanoic acid, succinimidyl ester)
Acryloyl-X, SE (6-((acryloyl)amino)hexanoic acid, succinimidyl ester) is an amine-reactive molecule that enables crosslinking of acrylamide to proteins and other biomolecules containing amines.
