Protein Analysis Reagents
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Thermo Scientific™ POROS™ GoPure™ AAVX Pre-packed Column, 0.5 x 5 cm, 1 mL
Thermo Scientific™ POROS™ GoPure™ pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification.
Invitrogen™ Ionomycin, Calcium Salt
Ionomycin is an effective Ca2+ ionopohre that is commonly used to both modify intracellular Ca2+ concentrations and to calibrate fluorescent Ca2+ indicators when studying the regulatory properties of Ca2+ in cellular processes.
Thermo Scientific™ Slide-A-Lyzer™ G3 Dialysis Cassettes, 10K MWCO
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Thermo Scientific Slide-A-Lyzer™ G3 Dialysis Cassettes facilitate the rapid and trouble-free dialysis of sample volumes from 0.5 to 125 mL. Unlike standard flat tubing, these innovative devices do not require knots or clips that can lead to leaking and sample loss.
| MWCO | 10 kDa |
|---|---|
| Format | Cassette |
| Membrane | Cellulose Membrane, Dialysis |
| Green Features | Less waste |
| Purification Target | Buffer Exchange, Protein |
| Product Line | Slide-A-Lyzer |
Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin
Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.
| Cell Permeability | Cell-Impermeant |
|---|---|
| Content And Storage | Store at -20°C. |
| Product Type | NHS-PEG4-Biotin |
| Label or Dye | Biotin |
| Solubility | DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water |
| Chemical Reactivity | Amine |
| Product Line | EZ-Link |
Thermo Scientific™ Pierce™ Protein A/G Magnetic Beads
Immunoprecipitate or affinity purify antibodies (IgG) using Protein A/G coated magnetic particles for manual or robotic magnetic separation.
| Particle Size | 1 μm |
|---|---|
| Ligand Type | Protein A/G |
| Type | Magnetic Affinity Bead |
| Target | Antibody |
| Content And Storage | Upon receipt store at 4°C. Product shipped with an ice pack. |
| Protein Form | Recombinant |
| Form | Liquid |
| Product Line | Pierce |
Thermo Scientific™ Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)
Optimize applications where product integrity and risk minimization are paramount with this high-quality formulation of carbodiimide crosslinker.
| Content And Storage | Upon receipt store desiccated at -20°C. |
|---|---|
| Cleavable | No |
| Water Soluble | Yes |
| Molecular Weight (g/mol) | 191.7 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Carboxyl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Carbodiimide |
| Spacer Arm Length | 0.0 Å |
| Product Line | Pierce |
| PEGylated | No |
Thermo Scientific™ Pierce™ Fab Preparation Kit
Digest human or mouse IgG antibodies with immobilized papain protease and purify Fab fragments using Protein A agarose.
| Shipping Condition | Ambient |
|---|---|
| Content And Storage | Upon receipt store at -20°C protected from light and moisture. |
| Cleavable | By Thiols |
| Water Soluble | Yes |
| Form | Powder |
| Molecular Weight (g/mol) | 492.4 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Nonselective |
| Cell Permeability | No |
| Format | Standard, Single-use |
| Product Type | Crosslinker |
| Spacer | Medium (10 to 30 Å) |
| Spacer Arm Length | 18.2 Å |
| PEGylated | No |
Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Description | EDC |
| Cleavable | No |
| Water Soluble | Yes |
| Form | Powder |
| Molecular Weight (g/mol) | 191.7 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Carboxyl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Carbodiimide |
| Spacer Arm Length | 0.0 Å |
| PEGylated | No |
Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli
One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.
| Shipping Condition | Dry Ice |
|---|---|
| Blue/White Screening | Yes (lacZΔM15) |
| Cloning Unstable DNA | No |
| Cloning Methylated DNA | Yes (mcrA) |
| Transformation Efficiency Level | High Efficiency (>1 x 1010 cfu/μg) |
| High-throughput Compatibility | Low |
| Improves Plasmid Quality | Yes (endA1) |
| Preparing Unmethylated DNA | No |
| Plasmid | May be used for plasmids >20 kb |
| Reduces Recombination | Yes (recA13) |
| T1 Phage - Resistant (tonA) | No |
| Antibiotic Resistance Bacterial | Yes (Streptomycin) |
| Format | Tube |
| Product Type | Electrocompetent Cells |
| Species | E. coli (K12) |
| Product Line | One Shot |
| Contains F' Episome | No |
Thermo Scientific™ Micro BCA™ Reagent B (MB)
Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).
Invitrogen™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
| Product Type | Dye |
| Label or Dye | Alexa Fluor™ 488 |
| Emission | 517 nm |
| Excitation | 494 nm |
| Label Type | Alexa Fluor |
| Chemical Reactivity | Amine |
| Product Line | Alexa Fluor |
Invitrogen™ MAX Efficiency™ Stbl2™ Competent Cells
MAX Efficiency Stbl2 Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts.
Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit
Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters
| Substrate | D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Brij™-35, 30% Solution
Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.