Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli

Supplied at transformation efficiency of 1 x 10⁹ cfu/ μg supercoiled DNA that's ideal for high-efficiency cloning and plasmid propagation

Brand:  Invitrogen™ C404052

Product Code. 10082692

Description

Provides stable replication of high copy number plasmids. The genotype of TOP10 cells is similar to that of DH10B™ strain.

• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA—get better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA
• Bacterial/Yeast Strain: TOP10

Cloning, Transformation

Specifications

• Bacterial or Yeast Strain: TOP10
• Cloning Methylated DNA: Yes
• Competent Cell Type: Electrocompetent
• Format: One Shot
• Improves Plasmid Quality: Yes
• Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
• Propagating ccdB Vectors: Not for ccdB vector propagation
• Reduces Recombination: Yes
• Species: E. coli
• Transformation Efficiency Level: High Efficiency (> 10^9 cfu⁄µg)
• For Use With (Application): Transformation, Cloning

• Blue/White Screening: Yes
• Cloning Unstable DNA: Not Suitable for cloning unstable DNA
• F'episome (to make ssDNA): Lacks F' Episome
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Plasmid: May be used for plasmids > 20 kb
• Product Line: One Shot™
• Quantity: 20 reactions
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): No
• Transformation Efficiency: > 1x10^10
• Product Type Specs: E. coil

For Research Use Only. Not for use in diagnostic procedures.