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PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.
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1
Promotions Available

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells

EDGE
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Product Line TOPO
2

Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli

Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System

EDGE
3

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

EDGE
Promoter SP6
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
Bacterial or Yeast Strain TOP10
Cell Type Chemically Competent
4

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells

Promoter SP6
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Kanamycin (KanR), Zeocin™ (ZeoR)
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
5

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells

Promoter T7, SP6
Content And Storage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Cloning Method TOPO™-TA
Vector TOPO-TA Vectors
Product Line TOPO
6

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli

Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform

7
Promotions Available

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

8

Invitrogen™ pENTR™/SD/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli

Use highly efficient, 5-minute cloning strategy to directionally clone a blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System

9

Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System

10

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

11

Invitrogen™ pENTR™/SD/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector

12

Invitrogen™ pENTR™/TEV/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector

13

Invitrogen™ pENTR™/TEV/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector

14

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing

Promoter T7, T3
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
Bacterial or Yeast Strain TOP10
For Use With (Application) Chromatin Biology
Vector Blunt DNA Cloning Vectors
Product Line One Shot
Cell Type Chemically Competent
15

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing

Shipping Condition Dry Ice
Promoter T7, T3
Content And Storage Box 1:
• Topoisomerase I-activated pCR™4Blunt-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• T3, T7, M13 forward, and M13 reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
Vector TOPO Cloning Vectors
Product Line TOPO