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DNA Reagents

Chemical reagents and other products used for DNA-related techniques and processes such as PCR cloning, DNA sequencing, DNA cloning and mapping, mutagenesis, DNA synthesis, etc.; includes kits, columns, buffers, labelling technology, and others.
DNA Sequencing (341)
PCR Cloning Kits (100)
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1
Promotions Available

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells

EDGE
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Product Line TOPO
2
Promotions Available

Applied Biosystems™ BigDye™ Terminator v1.1 Cycle Sequencing Kit

This BigDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

EDGE
Product Type Sequencing Buffer
For Use With (Equipment) SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐Well and 384‐Well Thermal Cyclers
Technique Fluorescent Dye Terminator Sequencing
Template Compatibility BAC DNA, Plasmid DNA (≤15 Kb), PCR Amplicons, Single Stranded DNA
Product Line BigDye™ Terminator
3
Promotions Available

Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

EDGE
Shipping Condition Ambient, Wet Ice, Dry Ice
Content And Storage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Workflow Step DNA Assembly
Number of Fragments Up to 6 fragments
Product Type HiFi Master Mix
Control Type Positive Control
Cloning Method Seamless cloning
For Use With (Application) Seamless Cloning & Genetic Assembly
Product Line GENEART
4

Applied Biosystems™ GeneScan™ 1000 ROX™ dye Size Standard

The GeneScan™ 1000 ROX™ Size Standard is an internal lane size standard developed for use with Applied Biosystems™ fluorescence-based DNA electrophoresis systems.

EDGE
5
Promotions Available

Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

Format 12-well Strips,8-well Strip,96- or 384-well Plate
Product Type Sequencing Buffer
For Use With (Equipment) SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
Technique Fluorescent Dye Terminator Sequencing
Product Line BigDye™ Terminator
6
Promotions Available

Invitrogen™ Collibri™ Library Quantification Kit

Invitrogen™ Collibri™ Library Quantification Kit includes a ready-to-use master mix optimized for Illumina NGS library quantification and a library dilution buffer

EDGE
Shipping Condition Dry Ice
Polymerase Platinum™ II Taq Hot Start DNA Polymerase
GC-Rich PCR Performance Low
Content And Storage Store at -20°C.
Format Kit
Product Type Library Quantification Kit
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, QuantStudio™, ViiA™ 7 System, StepOne™ System, StepOnePlus™ System
Sample Type DNA,RNA
PCR Method qPCR
For Use With (Application) Illumina NGS Library Quantification
Test Time Standard
Product Line Collibri
Detection Method SYBR
7
Promotions Available

Thermo Scientific™ CloneJET PCR Cloning Kit

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

EDGE
Antibiotic Resistance Bacterial Ampicillin (AmpR)
Format Kit
Sample Type PCR Products
Cloning Method Restriction Enzyme⁄ MCS, Blunt PCR
For Use With (Application) PCR Cloning
Vector pJET1.2/blunt
Product Line CloneJET
8
Promotions Available

Thermo Scientific™ CloneJET™ PCR Cloning Kit with DH10B Competent Cells

An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any blunt or sticky-end DNA fragment can be cloned.

EDGE
Content And Storage Dry Ice (-80°C)
Description Bacterial/Yeast Strain: DH10B
For Use With (Application) Cloning (Blunt PCR, Restriction Enzyme ⁄ MCS)
9
Promotions Available

Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

EDGE
Promoter T7
Content And Storage Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers.

Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt PCR
Bacterial or Yeast Strain Not Included
Vector Blunt DNA Cloning Vectors
Product Line TOPO, Zero Blunt
10
Promotions Available

Thermo Scientific™ phiX174 RF1 DNA

Prepare DNA molecular weight standards with Thermo Scientific™ phiX174 RF1 DNA, a 5386 bp double-stranded closed circular plasmid.

EDGE
11

Applied Biosystems™ BigDye™ Terminator v1.1 & v3.1 5X Sequencing Buffer

This sequencing buffer is optimized for use with BigDye™ Terminator v1.1 and v3.1 Cycle Sequencing Kits.

EDGE
Shipping Condition Room Temperature
Buffer Sequencing Buffer
Content And Storage Store at 2°C to 8°C.
Form Liquid
Concentration 5X
For Use With (Application) Standard Sequencing
12
Promotions Available

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

EDGE
Promoter SP6
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
Bacterial or Yeast Strain TOP10
Cell Type Chemically Competent
13
Promotions Available

Thermo Scientific™ Lysis Solution for GeneJET Plasmid Miniprep Kit

Thermo Scientific GeneJET Lysis Solution is a component of the GeneJET Plasmid Miniprep Kit and may be purchased separately.

EDGE
14
Promotions Available

Applied Biosystems™ POP-7™ Polymer For 3500 Dx Series

Conveniently offered in easy to use pouch packages

EDGE
Product Type POP-7™ Polymer
For Use With (Equipment) 3500 Dx Series
Size 1 Kit
15
Promotions Available

Applied Biosystems™ VetMAX™ Xeno™ Internal Positive Control DNA

VetMAX™ Xeno™ Internal Positive Control (IPC) DNA serves as an internal positive control for the DNA purification process and helps monitor for the presence of PCR inhibitors in animal health molecular detection workflows. This is of particular importance when working with sample types that have a high level of inhibitors such as feces.

EDGE
Shipping Condition Dry Ice
Includes Internal Positive Control
Format Kit
Product Type Internal Positive Control DNA
For Use With (Application) Detection and Identification of Animal Disease
Species Not Species-Specific
Product Line VetMAX, Xeno