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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (144)
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1 – 15 of 118 results
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Invitrogen™ Cell Lysis Buffer II

Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.

EDGE
2

Invitrogen™ InstantOne ELISA Cell Lysis Buffer (5X)

Combine with Enhancer Solution (cat. IOES1) to make the 5x Cell Lysis Mix used in the InstantOne phospho ELISA kits. The 5x Cell Lysis Buffer contains a combination of detergents, phosphatase inhibitors, salts, and buffers. The Cell Lysis Mix can be applied to many cells and tissues.

3

Invitrogen™ ProcartaPlex™ Cell Lysis Buffer

Used to lyse cells under nondenaturing conditions. Invitrogen™ ProcartaPlex™ Cell Lysis Buffer is available in 20mL units that should be stored chilled (for continual use) or frozen (for longer periods of time.) Additional protease inhibitors can be added without any difficulties.

4

Invitrogen™ Human MMP1-Cell Lysate ELISA Kit

ELISA

Protein Family Cell Structure & Organelle Proteins
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Cell Lysate 20 μL
Assay Sensitivity 8 pg/mL
Research Discipline Extracellular Matrix & Cell Adhesion
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target MMP1
Specificity The antibody pair provided in this kit recognizes human MMP-1.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell Lysis Buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Interassay CV <12%
Gene Symbol CLG,CLGN
Storage Requirements 2°C to 8°C
Assay Range 24.69 to 18,000 pg/mL
Accession Number P03956
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 4312
Sample Type Cell Lysates
Synonym CLG, CLGN
5

Invitrogen™ Human FGF4 (Cell Lysates) ELISA Kit

ELISA

Protein Family Growth Factors & Receptors
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Cell Lysate 20 μL
Assay Sensitivity 50 pg/mL
Research Discipline Apoptosis
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target FGF4
Specificity The antibody pair provided in this kit recognizes human FGF-4.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell Lysis Buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Interassay CV <12%
Gene Symbol HBGF-4,HST,HST-1,HSTF1,K-FGF,KFGF
Storage Requirements 2°C to 8°C
Assay Range 24.69 to 18,000 pg/mL
Accession Number P08620
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 2249
Sample Type Cell Lysates
Synonym HBGF-4, HST, HST-1, HSTF1, K-FGF, KFGF
6

Invitrogen™ Rat VEGF-A-Cell Lysates ELISA Kit

ELISA

Protein Family Chemokine & Receptors
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Cell Lysate 20 μL
Assay Sensitivity 2 pg/mL
Research Discipline Oncology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target VEGF
Specificity The antibody pair provided in this kit recognizes rat VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell Lysis Buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Sufficient For 96 Assays
Calibration Range 2 pg/mL to 200 pg/mL
Interassay CV <12%
Gene Symbol VEGF-A,VEGF164,VPF,Vegf
Storage Requirements 2°C to 8°C
Assay Range 0.82 to 200 pg/mL
Accession Number P16612
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 83785
Sample Type Cell Lysates
Synonym VEGF-A, VEGF164, VPF, Vegf
7

Invitrogen™ Rat LIX-Cell Lysate (CXCL5) ELISA Kit

ELISA

Protein Family Chemokine & Receptors
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Cell Lysate 20 μL
Assay Sensitivity 15 pg/ml
Research Discipline Immunology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target CXCL5
Specificity The antibody pair provided in this kit recognizes rat LIX / CXCL5.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell Lysis Buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Sufficient For 96 assays
Calibration Range 15 pg/mL to 6000 pg/mL
Interassay CV <12%
Gene Symbol Cxcl5, Cxcl6
Storage Requirements 2°C to 8°C
Assay Range 8.23 to 6000 pg/mL
Accession Number P97885
Regulatory Status RUO
Product Type ELISA
Sample Type Cell Lysates
Synonym Cxcl5
8

Invitrogen™ Rat TIMP-1-Cell Lysates ELISA Kit

ELISA

Protein Family Cytokines & Receptors
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Cell Lysate 20 μL
Assay Sensitivity 4 pg/mL
Research Discipline Oncology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target TIMP1
Specificity The antibody pair provided in this kit recognizes rat TIMP-1.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell Lysis Buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Sufficient For 96 Assays
Calibration Range 4 pg/mL to 3000 pg/mL
Interassay CV <12%
Gene Symbol Timp1
Storage Requirements 2°C to 8°C
Assay Range 4.1 to 3000 pg/mL
Accession Number P30120
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 116510
Sample Type Cell Lysates
Synonym TIMP-1, Timp
9

Invitrogen™ Rat Fractalkine-Cell Lysates (CX3CL1) ELISA Kit

ELISA

Protein Family Chemokine & Receptors
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Cell Lysate 20 μL
Assay Sensitivity 5 pg/mL
Research Discipline Oncology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target CX3CL1
Specificity The antibody pair provided in this kit recognizes rat Fractalkine.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell Lysis Buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Sufficient For 96 Assays
Calibration Range 5 pg/mL to 2000 pg/mL
Interassay CV <12%
Gene Symbol Cx3c,Scyd1
Storage Requirements 2°C to 8°C
Assay Range 2.74 to 2000 pg/mL
Accession Number O55145
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 89808
Sample Type Cell Lysates
Synonym Cx3c, Scyd1
10

Invitrogen™ Rat IL-1 beta-Cell Lysates ELISA Kit

ELISA

Protein Family Cytokines & Receptors
Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol Il1b
Total Assay Time 4 hrs 45 mins
Sample Volume Cell Lysate 20 μL
Assay Sensitivity 80 pg/mL
Storage Requirements 2°C to 8°C
Research Discipline Oncology
Assay Range 68.59 to 50,000 pg/mL
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Accession Number Q63264
Regulatory Status RUO
Target IL-1 beta
Specificity The antibody pair provided in this kit recognizes rat IL-1 beta.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell Lysis Buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 24494
Sample Type Cell Lysates
Label or Dye HRP
Sufficient For 96 assays
11

Invitrogen™ Rat NGF beta-Cell Lysates ELISA Kit

ELISA

Protein Family Growth Factors & Receptors
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Cell Lysate 20 μL
Assay Sensitivity 15 pg/mL
Research Discipline Immunology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target NGF
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, TIMP-1, TNF-alpha.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell Lysis Buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Sufficient For 96 assays
Calibration Range 15 pg/mL to 15000 pg/mL
Interassay CV <12%
Gene Symbol Ngf
Storage Requirements 2°C to 8°C
Assay Range 20.58 to 15,000 pg/mL
Accession Number P25427
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 310738
Sample Type Cell Lysates
Synonym Tgfb
12

Invitrogen™ Human BMP-4-Cell Lysates ELISA Kit

ELISA

Protein Family Growth Factors & Receptors
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Cell Lysate 20 μL
Assay Sensitivity 15 pg/mL
Research Discipline Angiogenesis
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target BMP4
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, BMP-6, BMP-7, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell lysis buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 15 pg/mL to 6000 pg/mL
Interassay CV <12%
Gene Symbol BMP2B,BMP2B1,MCOPS6,OFC11,ZYME
Storage Requirements 2°C to 8°C
Assay Range 8.23 to 6000 pg/mL
Accession Number P12644
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 652
Sample Type Cell Lysates
Synonym BMP2B, BMP2B1, MCOPS6, OFC11, ZYME
13

Invitrogen™ Human MIP-3 alpha/CCL20 Cell Lysate ELISA Kit

ELISA

Protein Family Chemokine & Receptors
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Cell Lysate 20 μL
Assay Sensitivity 1.5 pg/mL
Research Discipline Inflammation
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target CCL20
Specificity The antibody pair provided in this kit recognizes human MIP-3 alpha.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell Lysis Buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 1.5 pg/mL to 600 pg/mL
Interassay CV <12%
Gene Symbol CKb4,Exodus,LARC,MIP-3-alpha,MIP-3 A,MIP3A,SCYA20,ST38
Storage Requirements 2°C to 8°C
Assay Range 0.8 to 600 pg/mL
Accession Number P78556
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 6364
Sample Type Cell Lysates
Synonym CKb4, Exodus, LARC, MIP-3-alpha, MIP-3 A, MIP3A, SCYA20, ST38