Invitrogen™ Rat LIX-Cell Lysate (CXCL5) ELISA Kit

Artikelnummer. 17125919

missing translation for 'mfr': Invitrogen™ ERCXCL5CLX5

Beschreibung

ELISA

The Rat LIX (CXCL5) ELISA quantitates Rt LIX in rat cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Rt LIX. Principle of the method The Rat LIX solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is an inflammatory chemokine that belongs to the CXC chemokine family. This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.

Spezifikation

• Accession Number: P97885
• Assay Range: 8.23 to 6000 pg/mL
• Assay Sensitivity: 15 pg/ml
• Calibration Range: 15 pg/mL to 6000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: Chemokine & Receptors
• Sample Type: Cell Lysates
• Specificity: The antibody pair provided in this kit recognizes rat LIX / CXCL5.
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Cxcl5, Cxcl6
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Immunology
• Sample Volume: Cell Lysate 20 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Cxcl5
• Target: CXCL5
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins