Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Thermo Scientific Chemicals HEPES free acid, 99+%, for molecular biology, DNase, Rnase and Protease free
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid, free acid, MP Biomedicals™
CAS: 10191-18-1 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00007533 InChI Key: AJTVSSFTXWNIRG-UHFFFAOYSA-N Synonym: N, N-Bis(2-hydroxyethyl)taurine,N, N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid PubChem CID: 73243 ChEBI: CHEBI:39045 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid SMILES: OCCN(CCO)CCS(O)(=O)=O
MES monohydrate, free acid, ≥99%, MP Biomedicals™
CAS: 4432-31-9 Molecular Formula: C6H13NO4S Molecular Weight (g/mol): 195.233 MDL Number: MFCD00149409 InChI Key: SXGZJKUKBWWHRA-UHFFFAOYSA-N Synonym: 2-[N-morpholino]ethanesulfonic acid PubChem CID: 78165 ChEBI: CHEBI:39005 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid SMILES: C1COCCN1CCS(=O)(=O)O
MOPS, free acid, 100.2%, MP Biomedicals™
CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N Synonym: 3-[N-Morpholino]propanesulfonic acid PubChem CID: 70807 ChEBI: CHEBI:44115 SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1
HEPES Free Acid, >99%, Molecular Biology Reagent, MP Biomedicals™
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid,N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) PubChem CID: 23831 ChEBI: CHEBI:42334 SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
Melford MOPS, Free Acid
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer.
Melford TES Free Acid
Product usually ships within 24 hours. Research or further manufacturing use only, not for food or drug use.
Melford HEPES, Free Acid
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
![Melford PIPES [Piperazine-N-N'-bis(2-ethanesulfonic acid)]](https://assets.fishersci.com/TFS-Assets/CCG/Melford-Laboratories/product-images/P40140-100.0.jpg-250.jpg)
Melford PIPES [Piperazine-N-N'-bis(2-ethanesulfonic acid)]
Product usually ships within 24 hours. Research or further manufacturing use only, not for food or drug use.
PIPES, 98%
CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N Synonym: pipes,1,4-piperazinediethanesulfonic acid,piperazine-n,n'-bis 2-ethanesulfonic acid,2,2'-piperazine-1,4-diyl diethanesulfonic acid,1,4-piperazinebis ethanesulfonic acid,unii-g502h79v6l,2-4-2-sulfoethyl piperazin-1-yl ethanesulfonic acid,piperazine-1,4-bis 2-ethanesulfonic acid,1,4-piperazine-diethanesulfonic acid,2,2'-piperazine-1,4-diyl bis ethanesulphonic acid PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
Invitrogen™ TAE (10X), RNase-free
Molecular biology grade, Invitrogen 10X TAE is supplied in a 1 L bottle. The buffer is certified RNase-free, economical, and ready-to-use.
TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™
Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT
Invitrogen™ 10X TBE (powder)
Invitrogen 10X TBE (Tris-Borate-EDTA Buffer) is thoroughly tested for RNase and DNase contamination, making it ideal for use in sensitive application.