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Invitrogen™ Zenon™ Mouse IgG1 Labeling Kit

Simplify your cell staining applications with Invitrogen Zenon™ Mouse Antibody IgG1 Antibody Labeling Kits.

£381.00 - £432.00


Products 16
Product Code Brand Quantity Excitation Emission Label or Dye Price Quantity & Availability  
Product Code Brand Quantity Excitation Emission Label or Dye Price Quantity & Availability  
10553093
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Invitrogen™
Z25000
50 Reactions 346 442 Alexa Fluor™ 350
£381.00
Each
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Estimated Shipment: 14-12-2021
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10433642
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Invitrogen™
Z25002
50 Reactions 495 519 Alexa Fluor™ 488
£382.00
Each
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Estimated Shipment: 13-12-2021
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10728663
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Invitrogen™
Z25004
50 Reactions 556 573 Alexa Fluor™ 546
£387.00
Each
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Estimated Shipment: 13-12-2021
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10655573
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Invitrogen™
Z25005
50 Reactions 555 565 Alexa Fluor™ 555
£392.00
Each
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Estimated Shipment: 14-12-2021
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10023072
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Invitrogen™
Z25006
50 Reactions 578 603 Alexa Fluor™ 568
£385.00
1 set
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In stock, ready to ship: 1
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10194942
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Invitrogen™
Z25007
50 Reactions 590 617 Alexa Fluor™ 594
£393.00
Each
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Estimated Shipment: 10-12-2021
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10749033
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Invitrogen™
Z25008
50 Reactions 650 665 Alexa Fluor™ 647
£397.00
Each
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Estimated Shipment: 10-12-2021
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10790374
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Invitrogen™
Z25011
50 Reactions 401 421 Alexa Fluor™ 700
£419.00
Each
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Estimated Shipment: 20-12-2021
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10442112
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Invitrogen™
Z25013
50 Reactions 401 421 Alexa Fluor™ 405
£410.00
Each
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Estimated Shipment: 13-12-2021
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10404022
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Invitrogen™
Z25021
10 Reactions 650 665 Alexa Fluor™ 647-R-Phycoerythrin
£416.00
Each
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Estimated Shipment: 14-12-2021
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10318502
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Invitrogen™
Z25041
50 Reactions 410 455 Pacific Blue&trade
£413.00
Each
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Estimated Shipment: 20-12-2021
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10710584
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Invitrogen™
Z25051
25 Reactions 650 660 APC (Allophycocyanin)
£432.00
Each
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Estimated Shipment: 13-12-2021
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10720584
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Invitrogen™
Z25052
50 Reactions not applicable not applicable Biotin-XX
£388.00
Each
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Estimated Shipment: 10-12-2021
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10482292
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Invitrogen™
Z25054
25 Reactions not applicable not applicable HRP (Horseradish Peroxidase)
£393.00
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Estimated Shipment: 20-12-2021
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10104952
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Invitrogen™
Z25055
25 Reactions 496 578 R-PE (R-Phycoerythrin)
£417.00
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Estimated Shipment: 14-12-2021
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10164992
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Invitrogen™
Z25060
3 x 10 μL 495, 555, 650 519, 565, 660 Alexa Fluor™ 488, 555, 647
£381.00
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Description

Description

Simplify your multicolor staining and flow cytometry experiments with our Zenon™ mouse IgG1 labeling kits. These antibody labeling kits are ideal for flow cytometry (e.g., FACS) experiments because they enable the simultaneous labeling of different target cells and tissues with differently labeled mouse monoclonal antibodies in a single staining protocol. Multiple Zenon™ antibody complexes may be prepared individually and used in a multicolor stain after using the Zenon™ blocking reagent.

Achieve fast, versatile, and reliable fluorophore-, biotin-, or enzyme-labeled primary antibodies with the Zenon™ mouse IgG1 labeling kits. These kits utilize Alexa Fluor fluorophores, biotin, Pacific Blue, or enzymes such as R-phycoerythrin and allophycocyanin, which are attached to monovalent, affinity purified Fab fragments. The Fab fragments, in turn, are directed against and bind with the Fc portion of IgG1 primary antibodies. Only a small amount of starting material is required, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Because the Zenon labeling method is based on immunoselectivity, it does not require the removal of exogenous proteins (such as serum) or amine-containing buffers from the target antibody, simplifying the process.
Zenon labeling technology greatly simplifies the use of multiple mouse-derived antibodies in the same staining protocol. Zenon tricolor labeling kits contain sufficient materials for 10 labeling reactions of each of three different fluorescent colors.

Important features of Zenon labeling technology:

• Labeled antibodies are typically ready to use in 10 minutes
• Requires only 1–20 µg primary antibody
• Simple—no purification required
• Flexible — choose from different fluorophores, biotin, HRP, alkaline phosphatase
• Multiplex with other mouse monoclonal antibodies simultaneously
• Can be used in a variety of applications including ICC, IHC, flow cytometry, and cell imaging.

Advantages of using Zenon antibody labeling kits:

Cost savings
Zenon antibody labeling kits offer a cost-conscious and reproducible method of tagging as little as 0.4 µg in 2 µL of primary antibody, with minimal waste of expensive or difficult-to-obtain antibodies, or excessive washing steps that pose the risk of product loss.
Sensitivity
Label your primary antibodies without compromising their antigen binding affinity: Zenon dye- and enzyme-labeled Fab fragments, which are targeted to the Fc tail, are affinity purified during their preparation to ensure high affinity and selectivity for the Fc portion of the corresponding primary antibody. The procedure for chemical labeling of the Zenon Fab fragments protects their Fc-binding site, resulting in more active labeling reagents.
Speed
No purification procedure is required prior to using Zenon Fab fragments in your laboratory applications. Formation of the Fab-antibody complex occurs in fewer than five minutes, followed by a five-minute blocking step. During this time, almost all the primary antibody in the mixture is labeled with the labeled Fab fragments.
Simplicity
The Fab-antibody complexes display fluorescence or enzymatic activity that is similar in intensity to that of directly labeled primary antibodies. Varying the extent of the antibody labeling is as simple as changing the amount of added Zenon labeling reagent during the reaction. Once the labeling complexes are formed, they can used immediately, without need for antibody purification.
Reliability
The Zenon Fab-antibody complex is stable and allows subsequent or simultaneous labeling of different target cells and tissues with different complexes. After staining, an aldehyde-based fixing step may be used to prevent the transfer of different labels between different primary antibodies, preserving the initial staining pattern.
Customization
We offer custom antibody conjugation services that are efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.