VISTA Monoclonal Antibody (MIH64), Super Bright™ 436, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15993394	Affymetrix eBioscience 62-1083-80	25 μg
15903404	Invitrogen™ 62-1083-82	100 μg

Description

Description: This MIH64 monoclonal antibody recognizes mouse VISTA also known as B7-H5 or PD-H1. This MIH64 antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells. Applications Reported: This MIH64 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MIH64 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1 μg/mL per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells. It is usually absent on B cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.

Specifications

• Antigen: VISTA
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene ID (Entrez): 74048
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: MIH64
• Applications: Flow Cytometry
• Conjugate: Super Bright 436
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: Q9D659
• Gene Symbols: VSIR
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: VSIR