Thermo Scientific™ TMTpro™ 32plex and TMTpro™ Deuterated Label Reagents

Product Code. 30197496

Brand: Thermo Scientific™ A40000839

Description

TMTpro deuterated label reagents enable multiplexing of up to 35 samples, allowing greater throughput for protein identification and quantitation using tandem mass spectrometry.

Thermo Scientific TMTpro label reagents are the next generation of tandem mass tags, designed to increase the level of sample multiplexing without compromising on protein identification and quantitation. TMTpro deuterated label reagents enable multiplexing of up to 35 samples, for even greater throughput for protein identification and quantitative analysis by tandem mass spectrometry (MS/MS).

Features of the TMTpro 32plex Label Reagent Set include:

• Multiplex—concurrent MS analysis of up to 35 samples derived from cells, tissues, or biological fluids
• Robust—increased multiplex capability results in fewer missing quantitative values among samples and enables confidence among replicates
• Efficient—amine-reactive, NHS ester-activated reagents help ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
• Optimized for high-resolution MS/MS analysis using Orbitrap platforms with data processing by Proteome Discoverer 3.2

After fragmentation, each TMTpro tag generates a reporter ion with unique mass (e.g., 126-135 Da) in the low-mass region of the high-resolution MS/MS spectrum that is used for multiplexed relative quantitation of protein abundance.

Advantages of TMTpro tags

The advantages of TMTpro tags include increased multiplexed relative quantitation, increased sample throughput, and fewer missing quantitative values among samples. With these capabilities, the TMTpro label reagents are suited for applications requiring higher multiplexing and fewer missing values, such as inhibitor dose response experiments, time course experiments, thermal shift assays, or single-cell analysis. When combined with the industry-leading high-resolution Orbitrap instruments and software, TMTpro reagents provide integrated total solutions for quantitative proteomic analysis.

Combining TMTpro non-deuterated label reagents with their deuterated counterparts allows multiplexing up to 35 samples. Control channels within each set (non-deuterated and deuterated) are necessary to compare data across sub-plex. TMTpro-134C and TMTpro-135CD reagents are offered for use as control channels in combination with the 32plex set. TMTpro-135CD reagent is offered separately to combine with TMTpro 18plex and TMTpro 16plex deuterated label reagents to achieve 35-sample multiplexing, including multiple controls for data analysis.

For 32plex experiments, use TMTpro 32plex Label Reagent Matched Set (Cat. No. A40000839) or TMTpro 16plex Deuterated Label Reagent Set (Cat. No. A40000817) and TMTpro 16plex Label Reagent Set (Cat. No. A44520).

For 34plex experiments use TMTpro 32plex Label Reagent Matched Set (Cat. No. A40000839) and TMTpro-134C and TMTpro-135CD Label Reagents (Cat. No. A40000853) or TMTpro 16plex Deuterated Label Reagent Set (Cat. No. A40000817), TMTpro 16plex Label Reagent Set (Cat. No. A44520), and TMTpro-134C and TMTpro-135CD Label Reagents (Cat. No. A40000853).

For 35plex experiments use TMTpro 16plex Deuterated Label Reagent Set (Cat. No. A40000817), TMTpro-135CD Label Reagent (Cat. No. A40000818), and TMTpro 18plex Label Reagent Set (Cat. No. A52045).

Related products

For high resolution analysis of TMTpro-labeled peptides, the recommended LC column for the Nanospray Flex source is the Acclaim PepMap 100 C18 LC Column (Cat. No. 164942, 164939). For the EASY-Spray source, the recommended LC column is the EASY-Spray PepMap Neo UHPLC Column (Cat. No. ES75500PN) or EASY-Spray HPLC Column (Cat. No. ES902 or ES903).

Specifications

• For Use With (Equipment): Mass Spectrometer
• Product Type: 32plex Label Reagent Matched Set
• Product Line: TMTpro™
• Content And Storage: • TMTpro-126 Label Reagent, 1 x 5 mg
  • TMTpro-127N Label Reagent, 1 x 5 mg
  • TMTpro-127C Label Reagent, 1 x 5 mg
  • TMTpro-128N Label Reagent, 1 x 5 mg
  • TMTpro-128C Label Reagent, 1 x 5 mg
  • TMTpro-129N Label Reagent, 1 x 5 mg
  • TMTpro-129C Label Reagent, 1 x 5 mg
  • TMTpro-130N Label Reagent, 1 x 5 mg
  • TMTpro-130C Label Reagent, 1 x 5 mg
  • TMTpro-131N Label Reagent, 1 x 5 mg
  • TMTpro-131C Label Reagent, 1 x 5 mg
  • TMTpro-132N Label Reagent, 1 x 5 mg
  • TMTpro-132C Label Reagent, 1 x 5 mg
  • TMTpro-133N Label Reagent, 1 x 5 mg
  • TMTpro-133C Label Reagent, 1 x 5 mg
  • TMTpro-134N Label Reagent, 1 x 5 mg
  • TMTpro-127D Label Reagent, 1 x 5 mg
  • TMTpro-128ND Label Reagent, 1 x 5 mg
  • TMTpro-128CD Label Reagent, 1 x 5 mg
  • TMTpro-129ND Label Reagent, 1 x 5 mg
  • TMTpro-129CD Label Reagent, 1 x 5 mg
  • TMTpro-130ND Label Reagent, 1 x 5 mg
  • TMTpro-130CD Label Reagent, 1 x 5 mg
  • TMTpro-131ND Label Reagent, 1 x 5 mg
  • TMTpro-131CD Label Reagent, 1 x 5 mg
  • TMTpro-132ND Label Reagent, 1 x 5 mg
  • TMTpro-132CD Label Reagent, 1 x 5 mg
  • TMTpro-133ND Label Reagent, 1 x 5 mg
  • TMTpro-133CD Label Reagent, 1 x 5 mg
  • TMTpro-134ND Label Reagent, 1 x 5 mg
  • TMTpro-134CD Label Reagent, 1 x 5 mg
  • TMTpro-135ND Label Reagent, 1 x 5 mg
  
  Store below -20°C.
• Label or Dye: Tandem Mass Tag™ (TMT) Labels
• Shipping Condition: Dry Ice
• Starting Material: Cell Lysate
• Final Product Type: Peptide
• Detection Method: Mass Spectrometry
• Workflow Step: Peptide Labeling and Quantitation
• Quantity: 1 x 5 mg (per tag)
• Form: Lyophilized Powder

For Research Use Only. Not for use in diagnostic procedures.