TLR4 Antibody (76B357.1) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
18737893	Novus Biologicals NB100-56566SS	0.025 mg
18747783	Novus Biologicals NB100-56566	0.1 mg

Description

TLR4 Monoclonal specifically detects TLR4 in Human, Mouse, Rat, Porcine, Bovine, Mammal samples. It is validated for Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, In vitro assay, Dot Blot, SDS-Page, Flow (Cell Surface), Flow (Intracellular), ELISA Capture (Matched Antibody Pair), CyTOF-ready, Knockout Validated, Knockdown Validated, Block/Neutralize, Dual RNAscope ISH-IHC.

Specifications

• Antigen: TLR4
• Concentration: 1.0 mg/mL
• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoassay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), In Situ Hybridization (ISH), In vitro Assay, KnockDown, Neutralization
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Signal Transduction, Toll Like Receptors
• Gene Accession No.: O00206
• Gene ID (Entrez): 7099
• Immunogen: This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564).
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: 76B357.1
• Dilution: Western Blot 1-3 ug/ml, Flow Cytometry, ELISA, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 5 ug/ml, Immunohistochemistry-Frozen 1:500, In vitro assay, Dot Blot, SDS-Page reported in scientific literature (PMID 33166339), Flow (Cell Surface), Flow (Intracellular), ELISA Capture (Matched Antibody Pair) 0.5 ug, Chromatin Immunoprecipitation (ChIP) 1:10-1:500, CyTOF-ready, Knockout Validated, KnockDown Validated, Block/Neutralize, Dual RNAscope ISH-IHC
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Pig, Bovine
• Gene Alias: 76B357.1, ARMD10, CD284, CD284 antigen, hTollhomolog of Drosophila toll, tlr4 76B357.1, TLR4 facs, TLR4 flow cytometry, TLR4 human, TLR4 ihc, TLR4 western blot, TOLL, toll-like receptor 4
• Gene Symbols: TLR4
• Isotype: IgG2b κ
• Purification Method: Protein G purified
• Test Specificity: The amino acid sequence used has 100% identity in human, chimp, baboon, Chinese hamster, and rat; 93% identity in bovine and pig; 85% identity in sheep and 78% identity in cat, horse and mouse. Human, Mouse, Rat. Ground squirrel reactivity reported by a customer review. Predicted cross-reactivity based on sequence identity: Chimp (100%), Baboon (100%), Chinese Hamster (100%), Bovine (93%), Pig (93%). Mammal reactivity reported in scientific literature (PMID: 25130694).

For Research Use Only