TLR4 Antibody (76B357.1) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Brand:  Novus Biologicals NB100-56566

Product Code. 18747783

Description

TLR4 Monoclonal specifically detects TLR4 in Human, Mouse, Rat, Porcine, Bovine, Mammal samples. It is validated for Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, In vitro assay, Dot Blot, SDS-Page, Flow (Cell Surface), Flow (Intracellular), ELISA Capture (Matched Antibody Pair), CyTOF-ready, Knockout Validated, Knockdown Validated, Block/Neutralize, Dual RNAscope ISH-IHC.

Specifications

• Antigen: TLR4
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1-3 ug/ml, Flow Cytometry, ELISA, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 5 ug/ml, Immunohistochemistry-Frozen 1:500, In vitro assay, Dot Blot, SDS-Page reported in scientific literature (PMID 33166339), Flow (Cell Surface), Flow (Intracellular), ELISA Capture (Matched Antibody Pair) 0.5 ug, Chromatin Immunoprecipitation (ChIP) 1:10-1:500, CyTOF-ready, Knockout Validated, KnockDown Validated, Block/Neutralize, Dual RNAscope ISH-IHC
• Gene Alias: 76B357.1, ARMD10, CD284, CD284 antigen, hTollhomolog of Drosophila toll, tlr4 76B357.1, TLR4 facs, TLR4 flow cytometry, TLR4 human, TLR4 ihc, TLR4 western blot, TOLL, toll-like receptor 4
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: The amino acid sequence used has 100% identity in human, chimp, baboon, Chinese hamster, and rat; 93% identity in bovine and pig; 85% identity in sheep and 78% identity in cat, horse and mouse. Human, Mouse, Rat. Ground squirrel reactivity reported by a customer review. Predicted cross-reactivity based on sequence identity: Chimp (100%), Baboon (100%), Chinese Hamster (100%), Bovine (93%), Pig (93%). Mammal reactivity reported in scientific literature (PMID: 25130694).
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2b κ

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoassay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), In Situ Hybridization (ISH), In vitro Assay, KnockDown, Neutralization
• Clone: 76B357.1
• Conjugate: Unconjugated
• Gene Accession No.: O00206
• Gene Symbols: TLR4
• Immunogen: This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564).
• Quantity: 0.1 mg
• Research Discipline: Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Signal Transduction, Toll Like Receptors
• Gene ID (Entrez): 7099
• Target Species: Human, Mouse, Rat, Pig, Bovine
• Form: Purified

For Research Use Only