Invitrogen™ TFF-3 Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15485133	Invitrogen™ EHTFF3	96 Tests
17834220	Invitrogen™ EHTFF3X10	10 x 96 Tests
17135569	Invitrogen™ EHTFF3X5	5 x 96 Tests

Description

The Human TFF-3 ELISA quantitates Hu TFF-3 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TFF-3. Principle of the method The Human TFF-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and are clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.

Specifications

• Accession Number: Q07654
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: ITF,P1B,TFI
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 1.5 ng/mL
• Conjugate: Biotin
• Protein Family: Other Proteins
• Specificity: This ELISA pair antibody detects human TFF-3. Other species not determined.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 7033
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Endocrinology
• Synonym: ITF, P1B, TFI
• Test Time: 1 hr. 20 min.