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Invitrogen™ TFF-3 Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EHTFF3X5
Additional Details : Weight : 0.33820kg
Description
The Human TFF-3 ELISA quantitates Hu TFF-3 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TFF-3. Principle of the method The Human TFF-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and are clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.Specifications
Q07654 | |
1.5 ng/mL | |
Solid-phase sandwich ELISA | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
5 x 96 assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
7033 | |
<12% | |
Chromogen, Biotinylated Detection Antibody, SAV-HRP, Pre-coated 96 well plates, Adhesive Plate Covers, Assay Diluent Concentrate, Standard, Stop Solution, Wash Buffer | |
5 x 96 Tests | |
Endocrinology | |
2°C to 8°C | |
Human | |
4 hr. 45 min. |
2.048 ng/mL to 500 ng/mL | |
1.5 ng/mL to 500 ng/mL | |
Biotin | |
Other Proteins | |
This ELISA pair antibody detects human TFF-3. Other species not determined. | |
Human | |
Colorimetric Microplate Reader | |
ITF,P1B,TFI | |
<10% | |
HRP | |
RUO | |
Plasma, 50 μ;L; Serum, 50 μ;L; Supernatant, 100 μ;L | |
ITF, P1B, TFI | |
1 hr. 20 min. |