Thermo Scientific™ Tango™ MTNR1A-bla U2OS Cells

Product Code. 10450174

Brand: Thermo Scientific™ K1669

Description

The Tango™ MTNR1A-bla U2OS cells contain the human Melatonin Receptor 1A (MTNR1A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ MTNR1A-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ MTNR1A-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.

Specifications

• Accession Number: NM_005958.3
• Assay Entry: Cell-based beta-arrestin recruitment
• Cell State: Dividing Cells
• Content And Storage: K1669 Tango™ MTNR1A-bla U2OS cells
  (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
  Includes:
  • Tango™ MTNR1A-bla U2OS cells
  • Tango™ MTNR1A-bla U2OS Assay Protocol
  • Certificate of Analysis
  The Tango™ MTNR1A-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
• Cytoplasmic Tail: Native Tail
• Detection Method: Fluorescence
• Gene Symbol: MTNR1A
• Population Status: Single Cell Clone Origin
• Product Line: GeneBLAzer™
• Protein Family: GPCR (G-Protein Coupled Receptors)
• Quantity: 8 x 10⁶ cells
• Readout: End Point
• Reporter Gene: BLA (Beta-Lactamase)
• Target Entry: MTNR1A
• Cell Line: U2OS
• System Type: Tango™
• Druggable Target: GPCR

For Research Use Only. Not for use in diagnostic procedures.