Thermo Scientific™ SulfoLink™ Immobilization Kit for Proteins, 2 mL

Product Code. 10678884

Brand: Thermo Scientific™ 44995

Description

Covalently immobilize cysteine-peptides or proteins (sulfhydryl-ligands) for affinity purification using iodoacetyl-activated agarose beads and columns.

Thermo Scientific™ SulfoLink Coupling Resin is iodoacetyl-activated for the covalent immobilization of sulfhydryl-containing peptides or proteins for affinity purification.

SulfoLink Coupling Resin is porous, crosslinked 6% beaded agarose that has been activated with iodoacetyl groups. When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.

Complete SulfoLink Immobilization Kits are available for peptides and proteins. Each kit contains all the reagents for preparing the respective peptide or protein sample and five columns containing 2mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification. A single-column Trial Kit for use with either peptides or proteins is also available, or the SulfoLink Coupling Resin can be purchased separately.

Highlights:

• Specific conjugation through sulfhydryl (-SH) groups – the iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds

• Separate kits optimized for peptides or proteins – kits include optimized reagents for preparing peptide or protein samples for efficient immobilization

• Fast – spin columns increase protocol speed; prepare and couple samples in 2 hours (peptides) to 3.5 hours (proteins)

• Flexible coupling conditions – use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction

• Easy-to-follow instructions – streamlined protocols for sample preparation, immobilization and affinity purification

• High capacity – immobilize 1 to 2mg peptide or 2 to 20mg protein per 2mL column of SulfoLink Coupling Resin

Includes:

Kits contain SulfoLink Resin Columns (2mL), protein or peptide preparation reagents (e.g., reducing agent), coupling buffer, wash solution, L-cysteine solution (for quenching) and desalting columns (protein kit only)

Recommended for:

Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation; Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification

Specifications

• Capacity (Metric): 2 mL
• Content And Storage: Sufficient For: Immobilizing 1 to 10 mg of antibody per column
  • SulfoLink Columns, 2 mL, 5 columns
  • SulfoLink Preparation Buffer, 7.5 mL
  • SulfoLink Coupling Buffer, 500 mL
  • Wash Solution, 120 mL
  • PBS Pack (makes 500 mL), 1 pack
  • 2-Mercaptoethylamine·HCl, 5 x 6 mg
  • L-Cysteine·HCl, 100 mg
  • Zeba Spin Desalting Columns, 5 mL, 5 columns
  • Column Accessories
  
  Store at 4°C protected from light.
• Product Line: SulfoLink™
• Quantity: 5 Column Kit
• Type: Immobilization Kit
• Stationary Phase: Haloacetyl
• Sufficient For: 2 to 20 mg of Antibody per Column
• Format: Spin Column
• Column Type: Affinity, Agarose Resin

Safety and Handling

Product Identifier

• SulfoLink Immobilization Kit for Proteins, 2 mL

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 1 B
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P271-Use only outdoors or in a well-ventilated area.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P403+P233-Store in a well-ventilated place. Keep container tightly closed.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : L-(+)-cysteine hydrochloride monohydrate, 2-Mercaptoethylamine Hydrochloride, Tris(2-carboxyethyl)ph

For Research Use Only. Not for use in diagnostic procedures.