Thermo Scientific™ Sulfo-SBED Biotin Label Transfer Reagent, No-Weigh™ Format

Chemically tag purified proteins at primary amines to covalently crosslink and transfer biotinylation to protein interaction binding partners.

Brand:  Thermo Scientific™ 33034

Product Code. 10127883

Description

Thermo Scientific™ Pierce Sulfo-SBED Biotin Label Transfer Reagent is a multifunctional reagent for labeling a purified protein and then covalently transferring the attached biotin tag onto specific interactors of that protein.

Ready-to-use kit eliminates the need to prepare the necessary buffers or purchase the critical reagents necessary to perform a label transfer experiment. Key reagents, Sulfo-SBED and DTT, are supplied in unique, no-weigh, single-use packaging.

Highlights:

• Sulfo-NHS ester reacts with primary amines at pH 7-9
• Aryl azide conjugates randomly when activated with UV light
• Cleavable disulfide bond allows biotin label transfer
• Discover protein interactions without radiolabeling

Includes:

No-Weigh Sulfo-SBED Label Transfer Reagent, 8 × 1mg; BupH Phosphate Buffered Saline, 1 Pk. (makes 500mL); Label Transfer Buffer (20X), 200mL (makes 4L); Streptavidin-HRP Conjugate, 0.1mg; No-Weigh Dithiothreitol (DTT), 8 × 7.7mg; Slide-A-Lyzer MINI Dialysis Units plus Float, 10K MWCO, 10-100μL, 10 units/Pk.

Specifications

• Description: Pierce Sulfo-SBED Biotin Label Transfer Reagent, No-Weigh Format
• Quantity: 8 x 1 mg
• Molecular Weight (g/mol): 879.98
• Chemical Reactivity: Amine-to-Nonselective

• Formulation: Sulfo-N-hydroxysuccinimidyl-2-(6-[biotinamido]-2-(p-azido benzamido)-hexanoamido) ethyl-1,3'-dithioproprionate; powder solid
• Sufficient For: 18mL of typical 1X (0.5mM) reaction solution (total), or 8 separate uses
• Solubility: Water

For Research Use Only. Not for use in diagnostic procedures.