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IBA Lifesciences Strep-Tactin™XT Buffer Set

Product Code. 17044437 Shop All IBA Lifesciences Products
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Unit Size:
1 set
Product Code. Quantity unitSize
17044437 1 Set 1 set
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Product Code. 17044437

Brand: IBA Lifesciences 21043000

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This item is not returnable. View return policy
You may only order up to the available stock. Where an alternative item has been identified it will be shown below

Description

Strep-Tactin™XT protein purification buffer set with wash, elution and regeneration buffer

Strep-Tactin™XT Buffer Set contains all buffers necessary for purification of Strep-tag™II or Twin-Strep-tag™ fusion proteins with Strep-Tactin™XT as well as for subsequent regeneration of the resin. 10x Buffer W is a 10x concentrated wash buffer for Strep-Tactin™ and Strep-Tactin™XT resins. It removes unbound molecules from the affinity chromatography matrix during Strep-tag™II and Twin-Strep-tag™ fusion protein purification. 10x Buffer BXT is a 10x concentrated Strep-Tactin™XT elution buffer containing biotin. Biotin is a water-soluble B-complex vitamin, also known as vitamin B7 or vitamin H. It binds to Strep-Tactin™XT with very high affinity but reversibly and, thereby, allows the efficient elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins from the resin. Please note that 10x Buffer BXT is not applicable for elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins from Strep-Tactin™, since biotin irreversibly binds to these resins, and, thus, Strep-Tactin™ cannot be regenerated. Buffer XT-R is a ready-to-use Strep-Tactin™XT regeneration buffer containing magnesium chloride for competitive displacement of biotin from the modified biotin-binding pocket of Strep-Tactin™XT after the elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins. Please note that the Strep-Tactin™XT Buffer Set is not applicable for Strep-Tactin™.

Specifications

Concentration 10 x concentrated
Kit Contents 100 mL 10 x Buffer W, 25 mL 10 x Buffer BXT, 250 mL Buffer XT-R
Shipping Condition room temperature
Form Reagent Solution
Quantity 1 Set

Safety and Handling

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Product Identifier
  • 1SET Strep-TactinXT Purification Buffer Set
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P264-Wash thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
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