IBA Lifesciences Strep-Tactin™ TACS Agarose

Product Code. 17267750

Brand: IBA Lifesciences 66350025

Description

50% suspension for traceless affinity cell and exosome isolation

• Strep-Tactin™ TACS Agarose contains agarose macrobeads coated with the streptavidin variant Strep-Tactin™.
• A special crosslinking permits the use for cell isolation and depletion approaches.
• It was developed for column-based Traceless Affinity Cell Selection (Fab-TACS™/Nano-TACS™).
• In this approach, Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin™ on the agarose matrix.
• After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin™ Agarose by biotin addition.
• In this way, the eluted cells are ready for further downstream applications.
• The same principle is applicable for exosome isolation (Fab-TACS™ for exosomes)
• Alternatively to Fab-Streps/Nano-Streps, it is possible to bind Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding) to this resin.
• This makes Strep-Tactin™ TACS Agarose also suitable for negative cell selection or depletion approaches.
• The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography.
• To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.

Specifications

• Binding Capacity: Biotin Binding Capacity > 40 nmol/ml resin
• Capacity (Metric): 50 mL
• Formulation: 50% suspension in buffer, pH 8: 100 mM Tris-HCl pH 8.0, 1 mM EDTA, 150 mM NaCl, 0.01% sodium azide
• Support Type: Crosslinked agarose
• For Use With (Application): For Packing of Gravity Flow Columns for Affinity Chromatographic Cell or Exosome Isolation

For Research Use Only.