LanthaScreen™ STAT3 GripTite™ Cell Line

LanthaScreen™ STAT3 GripTite™ Cell Line is activated by cytokines such as IL-6.

Brand:  LanthaScreen™ K1488

Product Code. 10104643

Description

• Gene Symbol: STAT3
• Alternate Agonists: IL-6
• Antagonists: IFN-alpha
• Reporter: GFP (EmGFP)
• Binding of IL-6 to its cell-surface receptors results in the activation of JAKs, which in turn phosphoactivate STAT3 proteins at a specific tyrosine residue (Y705).
• LanthaScreen™ STAT3 GripTite™ is a human cell line that constitutively expresses a GFP-STAT3 fusion protein.
• The JAK/STAT signaling pathway is known to be functionally intact in this cell line; therefore, the GFP-STAT3 fusion protein serves as a substrate for IL-6-inducible phosphorylation.
• Using this cell line, a lytic immunoassay has been developed in which the phosphorylation state of GFP-STAT3 is detected in cell lysates using a terbium-labeled anti-pY705-STAT3 antibody in a time-resolved FRET (TR-FRET) readout.
• The GFP-STAT3 DNA expression construct was transfected into the GripTite™ HEK 293 cell line using Lipofectamine™ 2000 transfection reagent, and the transfected cells were selected with blasticidin.
• This cell line is a clonal population isolated by flow cytometry using GFP fluorescence as a sorting marker.
• The assay utilizing this cell line is validated for EC50 and Z’-factors under optimized conditions using IL-6 as a ligand for JAK-mediated GFP-STAT3 phosphorylation.
• This assay has also been tested under variable experimental conditions, including cell plating density, stimulation time, DMSO tolerance, and assay equilibration time.
• An alternate mix-and-read assay format is also described.

Specifications

• Content And Storage: Store in liquid nitrogen immediately upon receipt, or thaw for immediate use
• For Use With (Application): Cytokines, Kinases
• Quantity: 1 Vial
• Type: Dividing Cells