Invitrogen™ SOX2 Recombinant Rat Monoclonal Antibody (Btjce), Alexa Fluor™ Plus 594, Invitrogen™

Rat Recombinant Monoclonal Antibody

Brand:  Invitrogen™ 740013TP594

Product Code. 30194970

Description

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Applications Tested: This Btjce antibody has been tested by immunohistochemistry in mouse brain tissue, immunocytochemistry and flow cytometric analysis in NTERA-2 cells. This may be used for immunocytochemistry at 2 μg/mL and for flow cytometry at 0.5 μg per test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 590 nm; Emission: 618 nm; Laser: Yellow Laser Filtration: 0.2 μm post-manufacturing filtered.

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

Specifications

• Antigen: SOX2
• Classification: Recombinant Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8
• Gene Accession No.: P48431, P48432
• Gene Symbols: SOX2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20674, 6657
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry
• Clone: Btjce
• Conjugate: Alexa Fluor Plus 594
• Gene: SOX2
• Gene Alias: ANOP3; cb236; Delta EF2a; lcc; MCOPS3; MCOPS3 (Microphthalmia Syndromic type 3); RGD1565646; sex determining region Y-box 2; SOX 2; SOX2; Sox-2; soxp; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-box 2; SRY-box 2; SOX2; SRY-box containing gene 2; SRY-box transcription factor 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2; wu:fb83g04; wu:fc14d07; ysb; zgc:65860; zgc:77389
• Host Species: Rat
• Quantity: 50 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG2a κ