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Thermo Scientific™ SMART Digest™ Dual Enzyme Columns for HDX-MS Analysis

Product Code. 18078081 Shop All Thermo Scientific Products
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Product Code. 18078081 Supplier Thermo Scientific™ Supplier No. 60160110
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Estimated Shipment: 24-09-2026
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Description

Thermo Scientific™ SMART Digest™ Dual Enzyme Columns for HDX-MS analysis provide fast, reliable digestion of proteins for HDX-MS analysis, helping to provide the sequence characterization of biotherapeutics.

Thermo Scientific SMART Digest Dual Enzyme columns containing pepsin and protease XIII provide fast, reliable digestion of proteins for for HDX-MS analysis helping to provide the sequence characterization of biotherapeutics.

SMART Digest Dual Enzyme columns for HDX-MS analysis help to provide the characterization of biotherapeutics.

The combination of enzymes are complementary, creating many short, overlapping N-terminal peptides for a high degree of sequence coverage.

Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. The optimum pH range for pepsin activity is 1.0–3.0.

Proteinase XIII cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.

TRUSTED_SUSTAINABILITY

Specifications

Type SMART Digest Pepsin/Protease XIII Dual Enzyme column
Description SMART Digest Pepsin/Protease XIII Dual Enzyme Column
Enzyme Pepsin & Protease XIII
Cleavage Pepsin: Cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. The optimum pH range for pepsin activity is 1.0–3.0.

Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
For Use With (Application) HDX-MS analysis
Column Format Immobilized enzyme
Diameter 2.1 mm
Flow Rate 10 to 500 μL/min
Max. Pressure 2500 psi (170 bar)
Length 30 mm
Mobile Phase Compatibility Phosphate buffer pH 4
Particle Size ˜20 μm
pH 2 to 6
Pore Size ° 1000 angstroms
Operating Temperature Range 4 °C to 50 °C
Storage Temperature RT for up to 2 weeks; 4 °C for extended period (refrigerate in MES pH 6)
Stationary Phase Immobilized enzyme
Packing Material Hydrophilic/coated DVB
Column Type Immobilized enzyme in PEEK cartridge
Technique BioLC
Stability 1 year
Weight 8 g
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