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Protein Digestion Chromatography Sample Preparation Kits

Protein Digestion Chromatography Sample Preparation Kits

Kits designed to be used in the digestion of protein samples in preparation for chromatographic analysis.

Cleavage

C-terminal side of lysine and arginine amino acids (15)
Pepsin: Cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. The optimum pH range for pepsin activity is 1.0–3.0.

Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH. (1)

Enzyme

Pepsin & Protease XIII (1)
Trypsin (15)

Type

SMART Digest Pepsin/Protease XIII Dual Enzyme column (1)
Soluble Trypsin Kit (1)
Soluble Trypsin Kit - 10 Pk. (1)
Soluble Trypsin Kit - Low pH (1)
Trypsin Kit (12)

Capture

None (15)

Format

Bulk Low pH Magnetic Resin (1)
Bulk Low pH Resin (1)
Bulk Magnetic Resin (4)
Bulk Non-magnetic Resin (2)
Resin in Pre-filled PCR Tubes (4)
Soluble Trypsin (3)

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Thermo Scientific Chromatography (14)
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Cleavage

C-terminal side of lysine and arginine amino acids (15)
Pepsin: Cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. The optimum pH range for pepsin activity is 1.0–3.0.

Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH. (1)

Type

SMART Digest Pepsin/Protease XIII Dual Enzyme column (1)
Soluble Trypsin Kit (1)
Soluble Trypsin Kit - 10 Pk. (1)
Soluble Trypsin Kit - Low pH (1)
Trypsin Kit (12)

Format

Bulk Low pH Magnetic Resin (1)
Bulk Low pH Resin (1)
Bulk Magnetic Resin (4)
Bulk Non-magnetic Resin (2)
Resin in Pre-filled PCR Tubes (4)
Soluble Trypsin (3)

Product Line

SMART Digest (14)

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Thermo Scientific™ SMART Digest™ Trypsin Kits

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Thermo Scientific™ SMART Digest™ Dual Enzyme Columns for HDX-MS Analysis

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