Invitrogen™ SiteClick™ Antibody Azido Modification Kit

Product Code. 16605435

Brand: Invitrogen™ S10900

Description

Create a label-ready site-specific azido-modified antibody without lengthy genetic modification using the SiteClick Antibody Azido Modification Kit.

Create a label-ready, site-specific, azido-modified antibody without lengthy genetic modification using the SiteClick Antibody Azido Modification Kit. Use this kit to carry out a two-step conjugation process over three days. SiteClick labeling uses enzymes to attach an azido moiety to the heavy chains of an IgG antibody, ensuring the antigen-binding domains remain unaltered.

Create a label-ready, site-specific, azido-modified antibody without lengthy and inefficient genetic modification using the SiteClick Antibody Azido Modification Kit. SiteClick labeling uses enzymes to specifically attach an azido moiety to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain unaltered for binding to the antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on the Fc region of essentially all IgG antibodies regardless of isotype and host species.

Once azido–modified, a variety of SiteClick sDIBO or DBCO alkyne labels are available for covalent attachment to the antibody via copper-free click chemistry under mild reaction conditions at your choice of scale. This provides the flexibility to choose different fluorescent labels for your antibody, attach another molecule via streptavidin, or attach your own molecule, such as an oligo, drug, or toxin via amine-reactive or amine-containing moieties depending on your assay. This site-specific modification using SiteClick labeling is a critical tool in the development of therapeutic antibodies, antibody-drug conjugates, biologics, and immunodiagnostic tests that require high specificity with reproducible labeling reactions and helps ensure that antibody performance and function are preserved after conjugation.

Features of the SiteClick Antibody Azido Modification Kit include:
• Contains everything required to azido-modify any species or isotype of IgG antibody
• Easy-to-follow step-by-step protocol
• Highly efficient, site-specific labeling preserves antibody function and results in high quality antibody conjugate
• Reproducible labeling chemistry with lot-to-lot consistency in degree of labeling
• Optimal method for site-specific labeling of therapeutic antibodies, antibody-drug conjugates, biologics, and immunodiagnostics

Custom SiteClick Antibody Labeling Service and sDIBO labels
If you have an antibody that is considered 'difficult to label' or has lost activity after labeling using a conventional method, please contact our custom service representatives to determine whether the SiteClick Antibody Labeling Service would be right for your antibody. We offer complete custom SiteClick antibody labeling services with the option of multiple detection molecules including biotin, Alexa Fluor dyes, Qdot fluorophores, R-PE, chelates for PET imaging, and many others.

Specifications

• Product Type: Antibody Azido Modification Kit
• Quantity: for 1 mg of Ab
• Content And Storage: Store at 2°C to 8°C. Do not freeze.
• Labeling Scale: 1 mg
• Shipping Condition: Wet Ice
• Product Line: SiteClick
• Chemical Reactivity: Azide
• Reactive Moiety: Azide
• Label or Dye: Alexa Fluor 488, Alexa Fluor 647, Alexa Fluor 555
• Color: Clear
• Labeling Target: Antibodies

For Research Use Only. Not for use in diagnostic procedures.