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R&D Systems™ Recombinant Mouse GITR Ligand/TNFSF18 (aa 47-173)
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Brand: R&D Systems™ 2177-GL-025
Additional Details : Weight : 0.00970kg
Description
Measured by its ability to stimulate mouse T cell proliferation in the presence of anti-CD3. The ED50 for this effect is 0.02-0.3 μg/mL in the presence of 10 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050 ).Specifications
| AAO89011 | |
| Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
| M.W. (Observed): 16-26 kDa, reducing conditions; M.W. (theoretical): 16 kDa | |
| 25 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. | |
| Activation-inducible TNF-related ligand, AITRL, AITRLMGC138237, GITRL, GITRLglucocorticoid-induced TNFR-related protein ligand, Glucocorticoid-induced TNF-related ligand, hGITRLGITR ligand, TL6AITR ligand, TNFSF18, tumor necrosis factor (ligand) superfamily, member 18, tumor necrosis factor ligand superfamily member 18 | |
| Unconjugated | |
| Reconstitute at 100 μg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.) | |
| GITR Ligand/TNFSF18 |
| Bioactivity | |
| 8995 | |
| GITR Ligand/TNFSF18 aa 47-173 | |
| Mouse myeloma cell line, NS0-derived mouse GITR Ligand/TNFSF18 protein Thr47-Ser173, with an N-terminal 10-His tag | |
| <0.10 EU / 1 μg of the protein by the LAL method. | |
| Tnfsf18 | |
| Recombinant | |
| >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |