Invitrogen™ ReadyProbes™ Reagent F-Actin Phalloidin Conjugates

Product Code.	Color	Excitation Wavelength Range	unitSize
15119325	Red-Orange	540/565	Each
14879680	Green	495/518	5mL

Product Code. 15119325 Supplier Invitrogen™ Supplier No. R37112

Description

Includes

2 x 2.5 mL dropper bottles

ActinRed™ and ActinGreen™ ReadyProbes™ reagents assist with bright, selective F-actin labeling in fixed cells and tissues. The ready-to-use dropper or dual-use bottle enables dropwise dispensing or direct pipetting for fast, flexible cytoskeleton imaging.

ActinGreen and ActinRed ReadyProbes Reagents are selective, room temperature-stable solutions, with high-affinity F-actin probe conjugated to bright, photostable, fluorescent dyes. ActinGreen 488 reagent uses bright Alexa Fluor 488 Phalloidin (Cat. No. A12379) packaged in a dropper bottle. ActinRed 555 reagent uses Rhodamine Phalloidin (Cat. No. R415) packaged in a dropper bottle. ActinRed 568 uses Cell Paint’s Alexa Fluor™ 568 Phalloidin (Cat. No. A12380), and ActinRed 647 Plus uses the very bright Alexa Fluor™ Plus 647 Phalloidin (Cat. No. A30107) both packaged in a dual-use dropper bottle.

ActinGreen and ActinRed ReadyProbes Reagents are selective, room temperature-stable solutions, with high-affinity F-actin probe conjugated to bright, photostable, fluorescent dyes. ActinGreen 488 reagent uses bright Alexa Fluor 488 Phalloidin (Cat. No. A12379) packaged in a dropper bottle. ActinRed 555 reagent uses Rhodamine Phalloidin (Cat. No. R415) packaged in a dropper bottle. ActinRed 568 uses Cell Paint's Alexa Fluor™ 568 Phalloidin (Cat. No. A12380), and ActinRed 647 Plus uses the very bright Alexa Fluor™ Plus 647 Phalloidin (Cat. No. A30107) both packaged in a dual-use dropper bottle.

These ready-to-use solutions combine excellent brightness with exceptional F-actin staining. Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals and low non-specific binding or binding to G-actin.

Features

• High-affinity staining of F-actin with exceptional specificity compared to antibody methods
• ActinGreen 488 Excitation/Emission: (495/518 nm), detected through standard GFP and FITC filter sets
• ActinRed 555 Excitation/Emission: (540/565 nm), detected through standard RFP and TRITC filters sets
• ActinRed 568 Excitation/Emission: (578/600 nm), detected through standard RFP and TRITC filters sets
• ActinRed 647 Plus Excitation/Emission: (650/668 nm), detected through standard Texas Red and Cy5 filters sets
• Use counterstain (i.e., DAPI or NucBlue ReadyProbes reagent) as desired

ReadyProbes format advantages

• Ready-to-use formulation - no need to dilute, weigh, or pipette
• Just use two drops (80 μL) per mL of medium to stain, incubate 30 minutes, wash, then image
• ActinRed 568 ReadyProbes Reagent and ActinRed 647 Plus ReadyProbes Reagent are supplied in a dual-use bottle that supports either direct pipetting for plate-based workflows or dropwise dispensing after installation of the included dropper tip and cap.
• Stable at room temperature—keep handy on the benchtop

Applications

• For fixed and permeabilized cells or tissue
• Fluorescence microscopy (IF/IHC/ICC)
• High content screening, high content imaging
• Flow cytometry

Suggestions for use

• All ActinRed and ActinGreen reagents may be added directly to fixed cells in full medium or buffer solutions

In most cases 2 drops/mL and an incubation time of 15–30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.

Specifications

• Color: Red-Orange
• Content And Storage:

  2 x 2.5 mL dropper bottles

  Store at ≤ 25°C.

• Excitation Wavelength Range: 540/565
• Dye Type: TRITC (Tetramethylrhodamine Isothiocyanate)
• For Use With (Equipment): Confocal Microscope, EVOS™, Floid™ Cell Imaging System, Fluorescence Microscope, Flow Cytometer, Floid™ Cell Imaging System
• Quantity: 2 dropper bottle(s) kit
• Detection Method: Fluorescence
• Description: ActinRed™ 555 ReadyProbes™ Reagent (Rhodamine phalloidin)
• Emission: Visible
• Form: Liquid
• Product Line: ActinRed, ReadyProbes
• Technique: Fluorescence Intensity
• Label Type: Fluorescent Dye
• Product Type: Phalloidin
• Sub Cellular Localization: Cytoskeleton
• Excitation: 540

Frequently Asked Questions (FAQs)

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

For Research Use Only. Not for use in diagnostic procedures.