Macherey-Nagel™ Protino Ni-TED Resin for His-tag Protein Purification

Product Code. 11922532

Brand: Macherey-Nagel™ 745200.5

Description

For Purification of Polyhistidine-Tagged Proteins, Batch Binding, Gravity Flow Column Chromatography, MPLC/FPLC Applications. The matrix of choice for highest protein purity as dry bulk material.

Protino™ NI-TED, the purer alternative for His-tag protein purification

• Chelating group TED, one protein binding site
• High stability against reducing/chelating agents
• Low metal leaching
• High protein purity
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices
• Dry resin, storage at room temperature

Protino™ NI-TED packed columns

• Ready to use pre-packed columns filled with dry Protino™ Ni-TED resin
• Buffers included

Protino™ NI-TED resin

• Dry bulk matrix
• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink well - highest flexibility
• Batch procedure, gravity flow column chromatography, FPLC™ applications
• Empty Protino™ columns available for use with Protino™ Ni-TED resin
• Empty 96 well receiver plates available for use with Protino™ Ni-TED resin For purification of polyhistidine-tagged proteins; batch binding, gravity flow column chromatography, MPLC/FPLC™ applications.

Specifications

• Binding Capacity: 10mg/g
• Binding Property: Batch
• Flow Rate: 0.5 to 1.0 mL/min./cm²
• Kit Contents: Buffers
• Matrix: Macroporous Silica
• Max. Operating Pressure: 145 psi
• Quantity: 5 g Dry Silica Bulk Resin
• For Use With (Application): For Purification of Polyhistidine-Tagged Proteins, Batch Binding, Gravity Flow Column Chromatography, MPLC/FPLC Applications
• Type: Resin
• Ligand Type: TED (tris-carboxymethyl ethylene diamine)

Safety and Handling

Hazard Category

• Respiratory sensitiser Category 1