missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. Macherey-Nagel™ Protino Glutathione Agarose 4B for GST-tag Protein Purification

Macherey-Nagel™ Protino Glutathione Agarose 4B for GST-tag Protein Purification

Product Code. 11962462
Change view
Click to view available options
Quantity:
10 mL
100 mL
Unit Size:
100mL
10mL
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
11962462 10 mL 10mL
11912302 100 mL 100mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
This item is not returnable. View return policy
Do not autoclave the gel.
Product Code. 11962462 Supplier Macherey-Nagel™ Supplier No. 745500.10

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy
Do not autoclave the gel.

Description

Best choice for cost effective GST-tag protein purification.

Protino™ Glutathione Agarose 4B is an affinity chromatography medium that enables fast, single-step purification of glutathione-S-transferase (GST) fusion proteins and other glutathione-binding proteins. Purification of fusion proteins from a whole cell lysate is based on the strong affinity of the GST moiety for glutathione, which is immobilized on agarose beads.

  • Highest performance–Equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns
  • Simply replace your current products—No optimization or protocol change necessary
  • Universal usage—Suitable for small proteins, large protein complexes, proteins with low expression rates universal use
  • Highest flexibility of applications—For batch binding, gravity-flow column chromatography, MPLC/FPLC™

Specifications

Bed Volume 10 mL
Binding Capacity 8mg/mL
Concentration 0.1 M acetate, pH 4, 0.1 M NaOH, 70% ethanol, or 6 M guanidine hydrochloride
Flow Rate 250 cm/hr.
Material Settled beaded agarose
Matrix 4% beaded agarose
Content And Storage 4°C to 8°C for up to 3 years
Particle Size 90 μm
Quantity 10 mL
For Use With (Application) Purification of GST-tagged proteins using affinity chromatography
Type Agarose
Sample Volume 0.2 to 1.0 mL/min. (with 1 mL bed volume/column with 6.6 mm I.D.), 0.5 to 5.0 mL/min. (with 10 mL bed volume/column with 16 mm I.D.)
Ligand Type Chelating ligand: Glutathione, linked via sulfur atom
Show More Show Less

Safety and Handling

compliance-icons
Product Identifier
  • Protino Glutathione Agarose 4B
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Supplemental information
  • MIXTURE LIST-Contain: ethanol (diluted < 20 %) (denatured with1%IPA/1%MEK, acc.2016/1867/EU)
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.