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  6. Macherey-Nagel™ Protino Glutathione Agarose 4B for GST-tag Protein Purification

Macherey-Nagel™ Protino Glutathione Agarose 4B for GST-tag Protein Purification

Product Code. 11912302
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Quantity:
10 mL
100 mL
Unit Size:
100mL
10mL
This item is not returnable. View return policy
Do not autoclave the gel.

Product Code. 11912302

Brand: Macherey-Nagel™ 745500.100

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This item is not returnable. View return policy
Do not autoclave the gel.

Description

Best choice for cost effective GST-tag protein purification.

Protino™ Glutathione Agarose 4B is an affinity chromatography medium that enables fast, single-step purification of glutathione-S-transferase (GST) fusion proteins and other glutathione-binding proteins. Purification of fusion proteins from a whole cell lysate is based on the strong affinity of the GST moiety for glutathione, which is immobilized on agarose beads.

  • Highest performance–Equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns
  • Simply replace your current products—No optimization or protocol change necessary
  • Universal usage—Suitable for small proteins, large protein complexes, proteins with low expression rates universal use
  • Highest flexibility of applications—For batch binding, gravity-flow column chromatography, MPLC/FPLC™

Specifications

Bed Volume 100 mL
Binding Capacity 8mg/mL
Concentration 0.1 M acetate, pH 4, 0.1 M NaOH, 70% ethanol, or 6 M guanidine hydrochloride
Flow Rate 250 cm/hr.
Material Settled beaded agarose
Matrix 4% beaded agarose
Content And Storage 4°C to 8°C for up to 3 years
Particle Size 90 μm
Quantity 100 mL
For Use With (Application) Purification of GST-tagged proteins using affinity chromatography
Type Agarose
Sample Volume 0.2 to 1.0 mL/min. (with 1 mL bed volume/column with 6.6 mm I.D.), 0.5 to 5.0 mL/min. (with 10 mL bed volume/column with 16 mm I.D.)
Ligand Type Chelating ligand: Glutathione, linked via sulfur atom
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Safety and Handling

compliance-icons
Product Identifier
  • Protino Glutathione Agarose 4B
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol (diluted < 20 %) (denatured with1%IPA/1%MEK, acc.2016/1867/EU)
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