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Cytiva Protein G HP SpinTrap™ Columns

Product Code. 11580644 Shop All Cytiva Products
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Quantity:
800 μL
Unit Size:
100g
This item is not returnable. View return policy

Product Code. 11580644

Brand: Cytiva 28903134

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This item is not returnable. View return policy

Description

Includes

16 prepacked Protein G HP SpinTrap columns, one plastic bottom cap removal tool, and instructions. Also included is a protocol, with no pretreatment of sample required, for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min.

For small-scale purification of antibodies or enrichment of proteins of interest in prepacked spin columns

  • Protocols for both classic elution and cross-link methods; designed for use with a standard microcentrifuge
  • Formatted for elution for both electrophoresis and LC-MS analysis workflows
  • Fast-binding kinetics and high capacity provide high yield
  • Prepacked with Protein G Sepharose™ High Performance Medium for coupling of antibodies of IgG subclasses
  • Easy scale-up with HiTrap™ Protein G HP prepacked columns

  • Matrix: Highly cross-linked agarose, 6%
  • Binding capacity: >1mg human IgG/column
  • Average particle size: 34μm
  • pH stability: 3 to 9 (long term); 2 to 9 (short term)
  • Column volume: 800μL
  • Max. sample loading volume: 600μL

TRUSTED_SUSTAINABILITY

Specifications

Product Type Protein G Sepharose HP SpinTrap spin column
Quantity 800 μL
Includes 16 Prepacked Protein G HP SpinTrap column, one Plastic botto m cap removal to ol and instructions, proto col
Ligand Type Recombinant Protein G lacking Albumin-binding region (2mg/mL medium)
pH Range 3 to 9 (long term), 2 to 9 (short term)
Particle Size 34μm
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4° to 8°C
Column Type Protein G Sepharose Spin Column
For Use With (Application) Small-scale purification of Antibodies or enrichment of Proteins of interest in prepacked spin column, electrophoresis or LC/MS

Safety and Handling

compliance-icons
Product Identifier
  • Protein G HP SpinTrap
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol
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