Thermo Scientific™ ProPac™ IMAC-10 HPLC Columns

Resolve target proteins using a single column in a high-resolution gradient run with ProPac IMAC-10 LC Columns.

Product Code	Brand	Particle Size	Diameter (Metric)	Length (Metric)	Column Format
11331823	Thermo Scientific™ 063272	10 μm	2 mm	50 mm	Analytical Column
11301843	Thermo Scientific™ 063274	10 μm	2 mm	250 mm	Analytical Column
11311843	Thermo Scientific™ 063276	10 μm	4 mm	50 mm	Analytical Column
11321843	Thermo Scientific™ 063278	10 μm	4 mm	250 mm	Analytical Column
11331843	Thermo Scientific™ 063280	10 μm	9 mm	250 mm	Analytical Column
11381853	Thermo Scientific™ 063615	10 μm	9 mm	50 mm	Analytical Column
11391853	Thermo Scientific™ 063617	10 μm	1 mm	50 mm	Analytical Column
11331863	Thermo Scientific™ 063667	10 μm	4 mm	50 mm	Loading Column
11311873	Thermo Scientific™ 063710	10 μm	9 mm	50 mm	Loading Column

Description

Resolve target proteins using a single column in a high-resolution gradient run with Thermo Scientific™ ProPac™ IMAC-10 LC Columns. These analytical and semi-preparative HPLC columns provides high-purity separations of metal-binding proteins and high loading capacity for protein purification applications. The use of small, nonporous resin particles results in narrow, high-efficiency peaks, with minimal band broadening due to dispersion. Use loading columns to charge the ProPac IMAC-10 LC columns but avoid contact of the metal solution with the system.

Tailored Specificity for IMAC Separation of Proteins

• Retention control by imidazole concentration or pH gradient
• Stable between pH 2 to 12, compatible with common protein purification reagents (nonionic detergents, reducing agents)
• Effective under native or denaturing HPLC conditions
• Wide range of metal-specific applications, including the separation of His-tagged protein aggregation variants, prion peptide analysis and separation of monoclonal antibodies
• Shipped free from any metal and ready to be charged with the metal of your choice.
• Reusable columns with metal tailored specificity
• Resolve target proteins using a single column in a high resolution gradient run
• High purity separations of metal-binding proteins

Includes: 10μm, nonporous, polymeric beads coated with a hydrophilic layer, then grafted with poly(IDA) chains.

Specifications

• Max. Pressure: 3000 psi (207 bar)
• pH: 2 to 12
• Temperature: 60°C
• Packing Material: Spherical, Nonporous Polymer
• Phase: Affinity Separation

• Particle Size: 10 μm
• Pore Size: Nonporous
• Stationary Phase: Lysozyne Poly(IDA) Grafts
• Column Type: Separation of Metal-Binding Proteins