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Thermo Scientific™ POROS™ CaptureSelect™ C1-InhXL Affinity Matrix
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POROS CaptureSelect C1-InhXL Affinity Matrix is developed for the purification of human C1 esterase inhibitor from recombinant and plasma/serum sources. C1 esterase inhibitor is a protease inhibitor belonging to the serpin superfamily that inhibits the spontaneous activation of the complement system.
POROS CaptureSelect C1-InhXL affinity ligand is highly selective for human C1-inhibitor and coupled to the high-performing POROS matrix, allowing for an efficient and scalable purification process, with high purity and yield obtained in a single step. The large pore structure of the POROS beads enables higher flowrates and efficient binding of larger biomolecules.
Features of POROS CaptureSelect C1-InhXL Affinity Matrix include:
- Efficient purification of C1 esterase inhibitor with high yield
- Mild pH elution condition to retain activity
- High binding capacity
- Excellent scalability
- Non-animal derived
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics:
- Matrix: cross-linked poly(styrene-divinylbenzene)
- Average particle size: 50 μm
- Ligand: POROS CaptureSelect C1-InhXL affinity ligand
- Ligand coupling method: epoxide coupling
- Binding capacity:∼3–5 g C1-inhibitor/L resin
- Elution condition: 50 mM sodium acetate, 1.0 M MgCl2 , pH 5.0
- Regeneration conditions: acidic stripping condition with 0.5 M acetic acid, pH 2
- Mechanical resistance: 100 bar (1450 psi, 10 MPa)
- Formulation buffer: 20% (v/v) ethanol
Specifications
Specifications
| Product Line | CaptureSelect |
| Type | Affinity Matrix |
| Quantity | 10 mL |
| Form | Resin Slurry |
| Particle Size | 50 μm |
| Binding Capacity | ∼3 to 5 g/L |
| Ligand Type | C1-InhXL affinity ligand |
| Matrix | Cross-linked poly(styrene-divinylbenzene) |
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