PlGF-1 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen 500-P226BT-1MG

Product Code. 17875273

Description

AA Sequence of recombinant protein: MLPAVPPQQW ALSAGNGSSE VEVVPFQEVW GRSYCRALER LVDVVSEYPS EVEHMFSPSC VSLLRCTGCC GDENLHCVPV ETANVTMQLL KIRSGDRPSY VELTFSQHVR CECRPLREKM KPERCGDAVP RR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PlGF-1. Anti-Human PlGF-1-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hPlGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PlGF (500-P226) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hPlGF. Western Blot: To detect hPlGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPlGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P226BT-1MG will be provided as 2 x 500 μg

PlGF-1 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PlGF-1 is expressed in placental tissues, the colon, and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor, and stimulates endothelial cell proliferation and migration.

Specifications

• Antigen: PlGF-1
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: PGF
• Gene Symbols: PGF
• Immunogen: E.coli-derived, 29.7 kDa Recombinant Human PlGF-1
• Quantity: 2 x 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P49763
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 5228
• Content And Storage: -20°C