Thermo Scientific™ Pierce™ TRITC Rhodamine Dyes and Labeling Kits
Label antibodies, proteins and other molecules for fluorescence imaging (ex 546; em 572-615) using these activated derivatives of red-fluorescent rhodamine dye.
Brand: Thermo Scientific™ 46406
Code : PN
Additional Details : CAS Number : 27174-71-6 Weight : 1.81440kg
DescriptionRhodamine dyes are used in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. TRITC, NHS-rhodamine and Texas Red™ Sulfonyl Chloride are reactive towards primary amine groups on proteins, peptides and other biomolecules. Rhodamine dyes are often a mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.
- Label and purify antibody in about one hour Efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification and removal of non-reacted dye with excellent protein recovery
NHS-Rhodamine Antibody Labeling Kit (Mfr. No. 53031)
NHS-Rhodamine, 3 vials; Borate Buffer, 1mL; Purification Resin, 5mL; Spin Columns, 6 each; Collection Tubes, 12 each
|N-hydroxysuccinimide ester activated rhodamine fluorescent dye|
|Approx. 12 uses to label a total of 316mg of IgG using typical conditions|