Thermo Scientific™ Pierce™ TCEP-HCl, No-Weigh™ Format

Product Code.	Quantity	unitSize
10530434	1 g	1g
11856784	10 g	10g
15780329	10 x 1 mg	Pack of 10

Product Code. 10530434 Supplier Thermo Scientific™ Supplier No. 20490

Description

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

Thermo Scientific™ Pierce TCEP-HCl is pure, crystalline Tris(2-carboxyethyl)phosphine hydrochloride, a thiol-free compound that is highly effective at reducing protein and peptide disulfide bonds, as well as being odorless and stable.

Highlights:

• Reduces even the most stable water-soluble alkyl disulfides
• Effective at room temperature and pH 5 in less than five minutes
• Does not require removal before reaction with maleimide or haloacetyl compounds
• Water solubility of 310g/L
• Resistant to air oxidation; nonvolatile and nonreactive toward other functional groups in proteins

Specifications

• Content And Storage: Upon receipt store at room temperature in sealed container to prevent oxidation. Product shipped at ambient temperature.
• Product Line: Pierce
• Form: Solid
• Quantity: 1 g
• Solubility: Water
• Product Type: Disulfide Reducing Reagent

Frequently Asked Questions (FAQs)

Are other TCEP-based products available besides Immobilized TCEP Disulfide Reducing Gel?

Yes. We offer TCEP-HCl (1g solid, Cat. No. 20490, 10g solid, Cat. No. 20491), TCEP-HCl 10 x 1 mg No-weigh format, Cat. No. A35349 and TCEP Solution, Neutral pH (5 mL of stable 500 mM TCEP, Cat. No. 77720).

Can other reducing agents other than DTT or BME be used to reduce proteins prior to electrophoresis? For example, what about TCEP (Tris Carboxy Ethyl Phosphene)?

TCEP, Tris Carboxy Ethyl Phosphene is an alternative sulfhydryl reducing agent for protein samples. It is an extremely potent and effective reducing agent for particularly ‘difficult' proteins. It is compatible with the Tris-Glycine gels and NuPAGE gels. It should be added to the sample buffer for these systems. 20 mM final (maximum) concentration is sufficient for samples. You may add alkylating agents, e.g. Iodine (50 mM Iodoacetic acid), to prevent re-forming of S-S bonds but it is not necessary. Do not heat because this will hydrolyze much of your sample. Instead let the sample sit for several minutes at RT and then load.

Safety and Handling

Product Identifier

• TCEP HCl, Tris(2-Carboxyethyl) Phosphine Hydrochloride

Signal Word

• Danger

Hazard Category

• Serious eye damage/eye irritation Category 1
• Skin corrosion/irritation Category 1 B

Hazard Statement

• H314-Causes severe skin burns and eye damage.

Precautionary Statement

• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.

Supplemental information

• MIXTURE LIST-Contain: Tris(2-carboxyethyl)phosphonium chloride

For Research Use Only. Not for use in diagnostic procedures.