Thermo Scientific™ Pierce™ Sulfhydryl Addition Kit

Product Code. 10360924

Brand: Thermo Scientific™ 23460

Description

Modify protein primary amines to create free sulfhydryl groups for labeling, crosslinking or immobilization techniques; complete kit with SATA reagent and hydroxylamine.

Thermo Scientific™ Pierce Sulfhydryl Addition Kit is a complete set of reagents, including SATA, to enable modification of primary amines to free sulfhydryl groups for use in heterobifunctional crosslinking techniques.

Highlights:

• Reacts with primary amines to add protected sulfhydryls
• An easy deprotection step generates a free sulfhydryl group
• Thermo Scientific™ Sulfhydryl Addition Kit contains all necessary components for thiolation, deprotection, purification and quantitation

Specifications

• Chemical Reactivity: Amine
• Content And Storage: Sufficient For: One modification experiment with 0.5 to 5 mg of purified protein
  • SATA, 2 mg
  • Hydroxylamine·HCl, 5 mg
  • Conjugation Buffer Stock (10X), 20 mL
  • PBS pack (makes 500 mL), 1 pack
  • Dimethylformamide (DMF), 1 mL
  • Dextran Desalting Column, 1 x 5 mL
  • Column Extender, 1
  • Ellman's Reagent (DTNB), 2 mg
  • Cysteine·HCl, 20 mg
  
  Store at 4°C.
• Label or Dye: Sulfhydryl Group
• Label Type: Conjugating Groups
• Product Line: Pierce™
• Crosslinker Type: Heterobifunctional
• Reactive Moiety: Active Ester, Succinimidyl Ester, NHS Ester
• Spacer: Short (<10 Å)
• Form: Powder
• Reagent Type: SATA
• Quantity: 1 Reaction
• Solubility: DMF, DMSO
• Product Type: Crosslinker

Safety and Handling

Product Identifier

• Pierce Sulfhydryl Addition Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Acute toxicity Category 4
• ACUTE AQUATIC Acute 1
• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 2
• Substances and mixtures corrosive to metals Category 1
• Reproductive toxicity Category 1B
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1
• Specific target organ toxicity Category 1
• Specific target organ toxicity Category 3

Hazard Statement

• H290-May be corrosive to metals.
• H301-Toxic if swallowed.
• H312-Harmful in contact with skin.
• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H335-May cause respiratory irritation.
• H351-Suspected of causing cancer.
• H360D-May damage the unborn child.
• H373-May cause damage to organs.
• H400-Very toxic to aquatic life.

Precautionary Statement

• P101-If medical advice is needed, have product container or label at hand.
• P102-Keep out of reach of children.
• P103-Read label before use.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : disodium hydrogenorthophosphate, L-Cysteine, hydrochloride,3,3'-dithiobis [6-nitrobenzoic] acid, N,

For Research Use Only. Not for use in diagnostic procedures.