Thermo Scientific™ Pierce™ Phosphoprotein Enrichment Kit

Purify (enrich) phosphorylated proteins from mammalian cell and tissue lysates using phospho-specific IMAC affinity resin cartidges or kit.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 90003

PHOSPHOPROT ENRICHM (10 COLUMN)

UNSPSC: 41106510

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10320984

Quantity Price
1 £ 413.0 / 1 set
Estimated Shipment date
from Supplier 20-12-2016
Add to basket


Description and Specification

Specification

Description Pierce Phosphoprotein Enrichment Kit, 1mL
Formulation Multi-component kit with 10 x 1mL resin columns
Kit Contents CHAPS Detergent, 1g
Quantity 10 column kit
Sufficient For Purifying 10 protein samples each containing up to 4mg total protein (400ug phosphoprotein)
Support Type Agarose

The kit is an effective system for isolating the phosphoprotein fraction of mammalian cell and tissue lysates. The included lysis-binding buffer and proprietary affinity resin produce superior yields with negligible non-specific binding. The kit uses a convenient spin column format, and the resulting phosphorylated protein sample is compatible with downstream applications, including Western blotting, mass spectrometry (MS), 2D-PAGE, and protein arrays. The affinity resin is also available as pre-packed chromatography cartridges for use with automated liquid chromatography (LC) systems.

Highlights:

  • Specific – low nonspecific protein contamination from complex biological samples, such as cell culture lysate and mouse tissue extract

  • Fast – easy spin format enables enrichment of phosphorylated proteins in less than 2 hours

  • Superior yield – achieves higher yields than other commercially available phosphoprotein isolation kits

  • Convenient format – complete kit offering pre-dispensed spin columns, buffers, reagents and ultrafiltration columns for enrichment

  • Compatible – downstream applications include mass spectrometry, Western blotting and 2D-PAGE

Documentation

Technical documents