Thermo Scientific™ Pierce™ Phosphoprotein Enrichment Kit
Purify (enrich) phosphorylated proteins from mammalian cell and tissue lysates using phospho-specific IMAC affinity resin cartidges or kit.
Brand: Thermo Scientific™ 90003
Code : PN
Additional Details : Weight : 0.10000kg
DescriptionThe kit is an effective system for isolating the phosphoprotein fraction of mammalian cell and tissue lysates. The included lysis-binding buffer and proprietary affinity resin produce superior yields with negligible non-specific binding. The kit uses a convenient spin column format, and the resulting phosphorylated protein sample is compatible with downstream applications, including Western blotting, mass spectrometry (MS), 2D-PAGE, and protein arrays. The affinity resin is also available as pre-packed chromatography cartridges for use with automated liquid chromatography (LC) systems.
Specific – low nonspecific protein contamination from complex biological samples, such as cell culture lysate and mouse tissue extract
Fast – easy spin format enables enrichment of phosphorylated proteins in less than 2 hours
Superior yield – achieves higher yields than other commercially available phosphoprotein isolation kits
Convenient format – complete kit offering pre-dispensed spin columns, buffers, reagents and ultrafiltration columns for enrichment
Compatible – downstream applications include mass spectrometry, Western blotting and 2D-PAGE
|Pierce Phosphoprotein Enrichment Kit, 1mL|
|CHAPS Detergent, 1g, Column Accessories, Elution Buffer, 60mL, Lysis/Binding/Wash Buffer, 325mL, Phosphoprotein Enrichment Columns, 1mL, 10 columns, Protein Concentrators, 9K MWCO, 7mL, 10 concentrators|
|Purifying 10 protein samples each containing up to 4mg total protein (400ug phosphoprotein)|
|Multi-component kit with 10 x 1mL resin columns|
|10 column kit|