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Thermo Scientific™ Pierce™ Organelle Enrichment Kits

Enrich for lysosomes, peroxisomes and nuclei from cells and tissue with these organelle-specific subcellular fractionation kits.

Brand:  Thermo Scientific™ 89839

Code : PN

Additional Details : Weight : 2.72160kg

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Product Code. 10696574

  • £264.00 / 1 set


Estimated Shipment
29-11-2017
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Description

Description

These three organelle-specific kits provide the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation. Subcellular fractionation simplifies otherwise complex protein mixtures that result from whole cell lysis, thereby facilitating proteomic analysis of specific organelle targets and pathways. Each kit uses density gradient centrifugation to separate organelles from contaminating cellular structures. The isolated organelles may be used for a number of downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localization studies.

Highlights:

  • Efficient and easy to use – kit reagents and gradient centrifugation separate organelles from contaminating structures

  • Compatible – prepare samples for downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localization studies

  • Complete – kits contain sufficient material for 25 applications

Includes:

Organelle-specific Reagents A and B, Optiprep™ Cell Separation Media, and phosphate-buffered saline

Requires:

Ultracentrifuge, refrigerated microcentrifuge, vortex mixer, Dounce tissue grinder, sonicator

Specifications

Specifications

Lysosome Enrichment Reagent A, 90mL
Pierce Lysosome Enrichment Kit for Tissues and Cultured Cells
Cultured mammalian cells, Animal tissue
Four-component kit
230mL kit
25 samples each containing 50 to 200mg of cells or tissue
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