Thermo Scientific™ Pierce™ Organelle Enrichment Kits

Enrich for lysosomes, peroxisomes and nuclei from cells and tissue with these organelle-specific subcellular fractionation kits.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 89839

1 SET LYSOSOME ENRICHMENT KIT FOR TISSUES ANDcultured cells Store in fridge at 4°C

UNSPSC: 41116111

Code: PN

Additional Details:
Additional Details: Weight: 2.72160kg



Product Code. 10696574

Quantity Price
1 £ 264.0 / 1 set
EU Stock 2
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Description and Specification

Specification

Formulation Four-component kit
Description Pierce Lysosome Enrichment Kit for Tissues and Cultured Cells
Quantity 230mL kit
Source Material Cultured mammalian cells, Animal tissue
Kit Contents Lysosome Enrichment Reagent A, 90mL
Sufficient For 25 samples each containing 50 to 200mg of cells or tissue

These three organelle-specific kits provide the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation. Subcellular fractionation simplifies otherwise complex protein mixtures that result from whole cell lysis, thereby facilitating proteomic analysis of specific organelle targets and pathways. Each kit uses density gradient centrifugation to separate organelles from contaminating cellular structures. The isolated organelles may be used for a number of downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localization studies.

Highlights:

  • Efficient and easy to use – kit reagents and gradient centrifugation separate organelles from contaminating structures

  • Compatible – prepare samples for downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localization studies

  • Complete – kits contain sufficient material for 25 applications

Includes:

Organelle-specific Reagents A and B, Optiprep™ Cell Separation Media, and phosphate-buffered saline

Requires:

Ultracentrifuge, refrigerated microcentrifuge, vortex mixer, Dounce tissue grinder, sonicator

Documentation

Technical documents