Thermo Scientific™ Pierce™ Organelle Enrichment Kits
Enrich for lysosomes, peroxisomes and nuclei from cells and tissue with these organelle-specific subcellular fractionation kits.
Brand: Thermo Scientific™ 89839
Code : PN
Additional Details : Weight : 2.72160kg
DescriptionThese three organelle-specific kits provide the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation. Subcellular fractionation simplifies otherwise complex protein mixtures that result from whole cell lysis, thereby facilitating proteomic analysis of specific organelle targets and pathways. Each kit uses density gradient centrifugation to separate organelles from contaminating cellular structures. The isolated organelles may be used for a number of downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localization studies.
Efficient and easy to use – kit reagents and gradient centrifugation separate organelles from contaminating structures
Compatible – prepare samples for downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localization studies
Complete – kits contain sufficient material for 25 applications
Organelle-specific Reagents A and B, Optiprep™ Cell Separation Media, and phosphate-buffered saline
Ultracentrifuge, refrigerated microcentrifuge, vortex mixer, Dounce tissue grinder, sonicator
|Lysosome Enrichment Reagent A, 90mL, Lysosome Enrichment Reagent B, 90mL, OptiPrep Cell Separation Media, 50mL, PBS Pack (makes 500mL), 1 pack|
|Pierce Lysosome Enrichment Kit for Tissues and Cultured Cells|
|Cultured mammalian cells, Animal tissue|
|25 samples each containing 50 to 200mg of cells or tissue|