Thermo Scientific™ Mouse Embryonic Fibroblast Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits

Product Code. 15275723

Brand: Thermo Scientific™ 88290

Description

Ensure optimal yield and viability of fibroblasts from mouse embryos with the isolation and culturing reagents in this kit.

Ensure optimal yield and viability of fibroblasts from mouse embryos with the isolation and culturing reagents in the Thermo Scientific™ Pierce™ Mouse Embryonic Fibroblast Isolation Kit.

The Thermo Scientific Pierce Mouse Embryonic Fibroblast Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of fibroblasts from mouse embryos.

The Mouse Embryonic Fibroblast Isolation Kit has been designed to obtain high viability and purity of embryonic fibroblasts using validated reagents and an optimized procedure. The kit contains a tissue-specific dissociation enzyme and media formulated for primary cell culture that promotes the isolation growth of functional fibroblasts. In addition, MEF isolated and cultured with Pierce MEF Isolation Kit can be cryopreserved for future use with very minimal loss of viability.

Highlights:

• Optimized – procedure and reagents optimized for viability, yield, purity, and ease-of-use
• Yield – provides a 2-fold increase in yield compared to do-it-yourself methods and other commercial kits
• Functional – cultured fibroblasts express the appropriate biochemical markers

Includes:

• Complete kit includes Fibroblast Isolation Enzyme, Hanks' Balanced Salt Solution (HBSS), and DMEM for Primary Cell Isolation

Applications:

• Generation of immortalized cell lines from transgenic mice
• Feeder layer for stem cells
• IHC and IF studies

Product Details:

Because it uses an optimized tissue-specific dissociation enzyme, this kit isolates fibroblasts with greater yield and cell viability than cells isolated by existing methods. Isolated MEF express fibroblast-specific protein markers, confirming their viability and unperturbed metabolic function. After isolation, the mouse fibroblasts may be immortalized by continuous passage for the establishment of unique cell lines. They may also be used as a cell culture model for a diverse range of studies such as gene regulation and stem cell research [1,2,3,4].

Specifications

• Product Type: Cell Isolation Kit
• Content And Storage: Upon receipt store product at 2-80°C. Product is shipped on ice packs.
• Cell Type: Fibroblasts
• Target Species: Mouse
• Form: Solid
• Product Line: Pierce
• Quantity: 1 Vial

Sikkerhed og håndtering

Produktidentifikator

• Neuronal Isolation Enzyme (with Papain)

Signalord

• Danger

Farekategori

• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Faresætning

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H335-May cause respiratory irritation.

Sikkerhedssætning

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P310-Immediately call a POISON CENTER/doctor/.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplerende oplysninger

• MIXTURE LIST-Contains : papain

For Research Use Only. Not for use in diagnostic procedures.