Thermo Scientific™ Pierce™ Methyl-PEG-NHS-Ester Reagents

Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).

Brand: Thermo Scientific™

Manufacturer Part Number: 22685


UNSPSC: 12161502

Code: PN

Additional Details:
Additional Details: CAS Number: 642-91-1 Weight: 0.80000kg

Product Code. 11801325

Quantity Price
1 £172.00 / 100mg
Estimated Shipment date
from Supplier 07-11-2016
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Description and Specification


Capping Group Methyl
Chemical Reactivity Amine
Cleavable No
Description Pierce MS(PEG)12
Formulation 12-unit ethyleneglycol functionalized with methyl and succinimidyl ends; pale liquid
Molecular Weight 685.71
PEGylated Yes
Quantity 100mg
Sufficient For 146mL of typical 1X (1mM) reaction solution, or approx. 50 uses at 2mg reagent per use
Spacer Arm Length 44.9Å
Storage Requirements -20°C

MS(PEG)n is the abbreviation for a set of compounds having polyethylene glycol (PEG) spacers with methyl (—CH3) and amine-reactive NHS-ester groups at opposite ends. The unbranched, hydrophilic, discrete-length molecules have the form Methyl-PEGn-NHS Ester, where the subscript “n” denotes 4, 8, 12, or 24 ethylene glycol units. The N-hydroxysuccinimide (NHS) ester is spontaneously reactive with primary amines (—NH2), providing for efficient PEGylation of proteins, peptides and other amine-containing molecules or surfaces.


  • NHS-activated for efficient PEGylation of primary amines at pH 7-9; reaction of NHS-ester group results in formation of stable, irreversible amide bonds
  • Fully characterized PEGylation reagents with defined PEG chain lengths; molecules of discrete molecular weight for consistency of performance in protein-modification applications
  • Provided as a series of 4, 8, 12 and 24 ethylene glycol units, enabling modification procedures to be optimized for a specific application while retaining all the benefits associated with protein PEGylation
  • PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
  • Easy-to-follow instructions increase the likelihood of a successful outcome