Thermo Scientific™ Pierce™ Kinase Enrichment Kits and Probes

Desthiobiotin-ATP and -ADP probes to label, affinity purify and measure the active site of ATPases (kinases), including chaperones and metabolic enzymes.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 88311

X16 ActivX Desthiobiotin-ATP probe 12.6µg Store

UNSPSC: 41106510

Code: PN

Additional Details:
Additional Details: Weight: 2.26800kg



Product Code. 11822134

Quantity Price
1 £ 252.0 / Pack of 16
EU Stock 14
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Description and Specification

Specification

Description ActivX Desthiobiotin-ATP Probe
Formulation Desthiobiotin-ATP, MW 1259
Quantity 16 x 12.6µg
Sufficient For 16 pull-down assays

These products, based on ActivX ATP and ADP Probes, feature an amine-reactive nucleotide analog and a desthiobiotin (biotin analog) tag that facilitates selective labeling of lysines in the kinase active site and then subsequent enrichment and recovery of labeled protein. These features allow identification and profiling of target enzyme classes across samples or assessment of the specificity and affinity of enzyme inhibitors.

Highlights:

  • Specific – label only the conserved active-site lysines of nucleotide-binding proteins

  • Flexible – use for in vitro labeling of ATPase enzymes derived from cells or tissues

  • Compatible – use with Western blot or mass spectrometry (MS) workflows

Recommended for:

Profile small-molecule binding affinities and active-site inhibition in a dose-dependent manner; Identify dozens to hundreds of inhibitor targets and off-targets from tissues, cells and subcellular proteomes; Enrichment of enzymes based on function.