Thermo Scientific™ Hoechst 33342 Solution (20 mM)

High-quality Hoechst dye solution for fluorescent staining of DNA and nuclei in fixed- or live-cell imaging techniques.

Brand:  Thermo Scientific™ 62249

Product Code. 10150888

Description

Thermo Scientific™ Pierce Hoechst 33342 Fluorescent Stain is a high-quality solution of Hoechst dye for fixed- and live-cell fluorescent staining of DNA and nuclei in cellular imaging techniques.

Hoechst 33342 (2'-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole trihydrochloride trihydrate) is a cell-permeable DNA stain that is excited by ultraviolet light and emits blue fluorescence at 460 to 490nm. Hoechst 33342 binds preferentially to adenine-thymine (A-T) regions of DNA. This stain binds into the minor groove of DNA and exhibits distinct fluorescence emission spectra that are dependent on dye:base pair ratios.

Specifications

• Color: Blue
• Excitation Wavelength Range: 361 nm
• For Use With (Application): Fluorescent labeling
• Quantity: 5 mL
• Description: Hoechst 33342 Solution (20 mM)
• Form: Liquid
• Shipping Condition: Room Temperature
• SubCellular Localization: Nucleus

• Content And Storage: Upon receipt store at 4°C and protect from light.
• Dye Type: Cell-Permeant
• For Use With (Equipment): Fluorescence Microscope, High Content Instrument
• Detection Method: Fluorescence
• Emission: 497 nm
• Product Line: Pierce™
• Product Type: Nucleic Acid Stain

Safety and Handling

Product Identifier

• Hoechst 33342 Solution (20 mM)

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2

Hazard Statement

• H319-Causes serious eye irritation.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash thoroughly after handling.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental information

• MIXTURE LIST-Contains : 2'-(4'-ethoxyphenyl)-5- (4-methylpiperazin-1-yl) -2,5'-bis-1Hbenzimidazole trihydrochloride trihydra

For Research Use Only. Not for use in diagnostic procedures.